产品报价：2070元
更新时间：2018/4/11 9:25:23
产地：江苏
品牌：dldevelop
型号：DL-IGF1-Ra
厂商性质： 生产型,贸易型,
公司名称： 无锡市东林科技发展有限责任公司
郭蓓蓓 : (17809162583) (021-61431509)
（联系我时，请说明是在来宝网上看到的，谢谢！）大鼠胰岛素样生长因子1(IGF1) ELISA Kit DL-IGF1-Ra
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IGF1 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 2 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120μL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
SENSITIVITY
The minimum detectable dose of IGF1 is typically less than 30pg/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
大鼠胰岛素样生长因子1(IGF1) ELISA Kit DL-IGF1-Ra
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of IGF1.
No significant cross-reactivity or interference between IGF1 and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between IGF1 and all analogues, therefore, cross reactivity may still exist.
大鼠胰岛素样生长因子1(IGF1) ELISA Kit DL-IGF1-Ra
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF1 were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
STABILITY
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
