2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

别名:  o-Nitrophenyl β- D-galactopyranoside ONPG

• 经验分子式（希尔表示法） C₁₂H₁₅NO₈

• 分子量 301.25

•  Beilstein Registry Number 92207

•  EINECS 编号 206-716-1

•  MDL number MFCD00063255

•  PubChem Substance ID 24897461

Principle

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Reconstitution

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.