产品报价：询价
更新时间：2014/8/28 15:20:05
产地：美国
品牌：cell-lines-service
型号：HOS
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
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| HOS | ||||||||||||||||||||||||||||||||||||||||||||
| Depositor: | Riken | ||||||||||||||||||||||||||||||||||||||||||||
| Organism: | Homo sapiens (human) | ||||||||||||||||||||||||||||||||||||||||||||
| Ethnicity: | Caucasian | ||||||||||||||||||||||||||||||||||||||||||||
| Age/Stage: | 13 years | ||||||||||||||||||||||||||||||||||||||||||||
| Gender: | female | ||||||||||||||||||||||||||||||||||||||||||||
| Tissue: | bone | ||||||||||||||||||||||||||||||||||||||||||||
| Morphology: | A mixture of fibroblasts and epithelial like cells | ||||||||||||||||||||||||||||||||||||||||||||
| Celltype: | sarcoma, osteogenic | ||||||||||||||||||||||||||||||||||||||||||||
| Growth Properties: | monolayer | ||||||||||||||||||||||||||||||||||||||||||||
| Description: | HOS cells exhibit a flat morphology, low saturation density, low plating efficiency in soft agar and are sensitive to chemical and viral transformation. The cells express alkaline phosphatase under basal conditions (Clover J, 1994). | ||||||||||||||||||||||||||||||||||||||||||||
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). | ||||||||||||||||||||||||||||||||||||||||||||
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | ||||||||||||||||||||||||||||||||||||||||||||
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended | ||||||||||||||||||||||||||||||||||||||||||||
| Fluid Renewal: | 2 to 3 times weekly | ||||||||||||||||||||||||||||||||||||||||||||
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | ||||||||||||||||||||||||||||||||||||||||||||
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative | ||||||||||||||||||||||||||||||||||||||||||||
| Biosafety Level: | 1 | ||||||||||||||||||||||||||||||||||||||||||||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 D3S1358: 15 D21S11: 31.2 D18S51: ,14 Penta E: 7,12 Penta D: 9,10 D8S1179: 14 FGA: 24 CLS ? Cell Lines Service, 2011. | ||||||||||||||||||||||||||||||||||||||||||||
| Isoenzymes: | G6PD, B | ||||||||||||||||||||||||||||||||||||||||||||
| Viruses: | SMRV negative, as confirmed by Real-Time PCR | ||||||||||||||||||||||||||||||||||||||||||||
References: McAllister RM, Gardner MB, Greene AE, Bradt C, Nichols WW, Landing BH. Cultivation in vitro of cells derived from a human osteosarcoma. Cancer 27: 397-402, 1971. McAllister RM et al. Transformation and productive infection of human osteosarcoma cells by a feline sarcoma virus. Nat. New Biol. 230(17): 279-282, 1971. Rhim JS, Cho HY, Huebner RJ. Non-producer human cells by murine sarcoma virus. Int. J. Cancer 15(1): 23-29, 1975. Rhim JS et al. Characterization of non-producer human cells induced by Kirsten sarcoma virus. Int. J. Cancer 16: 840-849, 1975. Rhim JS. Characterization of sarcoma-positive, leukemia-negative (S+L-) human cells induced by the feline leukemia virus pseudotype of Moloney sarcoma virus. Proc. Soc. Exp. Biol. Med.167: 597-606, 1981. Rhim JS et al. Differential susceptibility of human cells to transformation by murine and avian sarcoma viruses. Proc. Soc. Exp. Biol. Med. 170: 350-358, 1982. Clover J, Gowen M. Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone 15(6): 585-591, 1994. Yee A et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. Sunjic SB et al. The influence of 4-hydroxy-2-nonenal on proliferation, differentiation and apoptosis of human osteosarcoma cells. Biofactors 24(1): 141-148, 2005. | |||||||||||||||||||||||||||||||||||||||||||||
