| Designation: | UM-SCC-14C | | Depositor: | CLS | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Gender: | male | | Tissue: | Mouth | | Morphology: | epithelial | | Celltype: | Squamous cell carcinoma | | Growth Properties: | monolayer | | Culture Medium: | DMEM:Ham’s F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Tumorigenic: | yes, in nude mice | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12
D5S818: 11,14
D7S820: 9,10
THO1: 6,8
TPOX: 8
vWA: 14,18
D3S1358: 15
D21S11: 29
D18S51: 15
Penta E: 7
Penta D: 12,16
D8S1179: 8,13
FGA: 20,21
CLS ? Cell Lines Service, 2011. | | Reverse Transcriptase: | negativ | | Viruses: | Negative: Sendai, Ektromelia, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler's GD VII, toolan's H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis | | Products: | keratin |
References:
Grenman R et al. Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution. Int J Cancer 44: 131-6, 1989.
Braakhuis BJ et al. Importance of pharmacodynamics in the in vitro antiproliferative activity of the antifolates methotrexate and 10-ethyl-10-deazaaminopterin against human head and neck squamous cell carcinoma. Biochem Pharmacol 46: 2155-61, 1993.
Ruiz van Haperen VW et al. Deoxycytidine kinase and deoxycytidine deaminase activities in human tumour xenografts. Eur J Cancer 29A:2132-7, 1993.
Ruiz van Haperen VW et al. Schedule dependence of sensitivity to 2',2'-difluorodeoxycytidine (Gemcitabine) in relation to accumulation and retention of its triphosphate in solid tumour cell lines and solid tumours. Biochem Pharmacol 48: 1327-39, 1994.
Kubler AC et al. [Clinical effectiveness of m-THPC-PEG in a new xenogenic animal tumor model for human squamous epithelial carcinomas]. Mund Kiefer Gesichtschir 5: 105-13, 2001.
Klaassen I et al. Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines. Int J Cancer 92: 661-5, 2001.
Reuther T et al. The RAG 2 mouse model for xenografted human oral squamous cell carcinoma. Contemp Top Lab Anim Sci 41: 31-5, 2002. |
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