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现在位置首页>医疗器械>体外诊断试剂>分子诊断试剂>HNO258 ,HNO258 Humane Larynx Karzinom Zell-Linie 300146,330146
HNO258 ,HNO258  Humane Larynx Karzinom Zell-Linie    300146,330146
HNO258 ,HNO258  Humane Larynx Karzinom Zell-Linie    300146,330146
  • HNO258 ,HNO258  Humane Larynx Karzinom Zell-Linie    300146,330146

HNO258 ,HNO258 Humane Larynx Karzinom Zell-Linie 300146,330146

产品报价:询价

更新时间:2014/8/28 12:04:59

地:美国

牌:cell-lines-service

号:HNO258

厂商性质: 生产型,贸易型,服务型,

公司名称: 北京百杰生物科技有限公司

产品关键词:

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李胜亮 : (13466675923) (13466675923)

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BITTE BEACHTEN

Für den Erwerb der HNSCC Zelllinien HNO41, HNO97, HNO210, HNO223 und HNO258 müssen bestimmte Voraussetzungen erfüllt sein:

Nur gemeinnützige Institutionen k?nnen diese Zelllinien über CLS Cell Lines Service GmbH erwerben. Bitte kontaktieren Sie uns.

Ein MTA muss unterzeichnet werden.

Ohne ein unterzeichnetes Bestellformular der CLS Cell Lines Service GmbH kann kein Versand erfolgen.

Designation:HNO258
Depositor:C. Herold-Mende
Organism:Homo sapiens (human)
Ethnicity:European
Age/Stage:62 years of age
Gender:Male
Tissue:Oral Cavity
Morphology:Epithelial
Celltype:Head and neck squamous cell carcinoma (HNSCC) of the oral cavity.
Growth Properties:Adherent
Culture Medium:DMEM supplemented with L-glutamine, 4.5 g/L glucose and 10% fetal bovine serum.
Subculturing:Remove spent medium and rinse using PBS without calcium/magnesium. Add fresh Accutase solution and let the culture sit at 37°C for 10 minutes. Collect the cells and dispense into new flasks. Comment: A general trypsin subculture routine may be used.
Split Ratio:An initial ration of 1:3 is recommended.
Fluid Renewal:2-3 times weekly
Freeze Medium:CM-1 (CLS order no. 800150, 50 ml)
Sterility:Tests for mycoplasma (DAPI, PCR), bacteria (PCR) and fungi were negative.
Biosafety Level:1
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8
THO1: 7
TPOX: 9,11
vWA: 16,17


References:

Vasvari GP, Dyckhoff G, Kashfi F, Lemke B, Helmke BM, Schirrmacher V, Plinkert PK, Beckhove P, Herold-Mende C. Combination of thalidomide and cisplatin in an HNSCC model results in an enhanced anti-angiogenic activity in vitro and in vivo. Int J Cancer 121: 1697-704, 2007.
Freier K, Hofele C, Knoepfle K, Gross M, Devens F, Dyckhoff G, Plinkert P, Lichter P, Herold-Mende C. Cytogenetic characterization of head and neck squamous cell carcinoma cell lines as model systems for the functional analyses of tumor-associated genes. J Oral Pathol Med. 2010 May;39(5):382-9. doi: 10.1111/j.1600-0714.2009.00864.x. Epub 2010 Feb 8.
COLO-60H
Depositor:CLS
Organism:Homo sapiens (human)
Age/Stage:73 years of age
Gender:Male
Tissue:Colon transversum adenocarcinoma.
Morphology:Epithelial
Celltype:Colon adenocarcinoma
Culture Medium:DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Fluid Renewal:Every 2 to 3 days
Freeze Medium:CM-1 (CLS order number 800125 or 800150)
Sterility:Tests for bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:not tested
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011.