| Designation: | CCF-STTG1 | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 68 years | | Gender: | Female | | Tissue: | Brain | | Morphology: | astrocytoma, grade IV | | Celltype: | astrocytic | | Culture Medium: | RPMI 1640 supplemented with 4.5g/L glucose, 2 mM glutamine and 10% fetal bovine serum (MG-72, CLS order number 820702). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:3 to 1:8 is recommended | | Fluid Renewal: | Every 2 to 3 days. | | Freeze Medium: | CM-1 (CLS order number 800125 or 800150) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | DNA Profile (STR): | Amelogenin: X, X
CSF1PO: 12
D13S317: 11, 13
D16S539: 11, 12
D5S818: 12, 13
D7S820: 10, 11
THO1: 7, 8
TPOX: 8, 11
vWA: 17
D3S1358: 16, 17
D21S11: 28, 29
D18S51: 15, 15
Penta E: 10, 10
Penta D: 11, 13
D8S1179: 13, 14
FGA: 20, 22
CLS ? Cell Lines Service, 2010. | | Antigen Expression: | HLA DR (on approx. 25% of cells) |
References:
In Vitro 19; 275,1983.
Fed.Proc. 43: 781, 1984.
Barna BP, Chou SM, Jacobs B, Ransohoff RM, Hahn JF, Bay JW. Enhanced DNA synthesis of human glial cell exposed to human leukocyte products. J Neuroimmunol 10(2): 151-158, 1985.
Krul ES, Tank J. Secretion of apolipoprotein E by an astrocytoma cell line. J Neurosci Res 32(2): 227-238, 1992.
Beaman L, Beaman BL. Differences in the interactions of Nocardia asteroides with macrophage, endothelial, and astrocytoma cell lines. Infect Immun 62(5): 1787-1798, 1994.
Thilakawardhana S, Everett DM, Murdock PR, Dingwall C, Owen JS. Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction. Neurobiol Aging 26(6): 813-823, 2005. |
| COLO-60H |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 73 years of age |
| Gender: | Male |
| Tissue: | Colon transversum adenocarcinoma. |
| Morphology: | Epithelial |
| Celltype: | Colon adenocarcinoma |
| Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | not tested |
| Designation: | SW-579 | | Depositor: | Leibovitz | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 59 years | | Gender: | male | | Tissue: | squamous cell carcinoma; thyroid | | Morphology: | epithelial | | Growth Properties: | monolayer | | Description: | According to sequencing and WB results this cell line is BRAF V600E wild type. | | Culture Medium: | RPMI 1640 medium supplemented with 2mm L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:5 to 1:10 is recommended | | Fluid Renewal: | 2 to 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | Biosafety Level: | 1 | | Tumorigenic: | yes, produces a grade III malignant spindle and giant cell tumor in nude mice | | DNA Profile (STR): | Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11
D5S818: 11
D7S820: 8,9
THO1: 8,9.3
TPOX: 8,10
vWA: 14,18
D3S1358: 15,18
D21S11: 29,31
D18S51: 15,17,18
Penta E: 11,12
Penta D: 9,12
D8S1179: 11,13
FGA: 21, 24
CLS ? Cell Lines Service, 2011. | | Antigen Expression: | blood type O; Rh+ | | Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0209 |
References:
Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977.
Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977.
Lin JD et al. Establishment of xenografts and cell lines from well-differntiated human thyroid carcinoma. J Surg Oncol 63: 112-8, 1996.
Yang DM et al. Effects of clodronate on cancer growth and Ca2+ signaling of human thyroid carcinoma cell lines. Anticancer Res 24: 1617-23, 2004.
Karadag A et al. Bone sialoprotein, matrix metalloproteinase 2, and alpha(v)beta3 integrin in osteotropic cancer cell invasion. J Natl Cancer Inst 96: 956-65, 2004.
Mitsiades CS et al. Fas signaling in thyroid carcinomas is diverted from apoptosis to proliferation. Clin Cancer Res 12: 3705-12, 2006. |
| Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011. |
