| Designation: | Detroit 562 | | Depositor: | CLS | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | adult | | Gender: | female | | Tissue: | pharynx; from metastatic site: pleural effusion | | Morphology: | epithelial | | Celltype: | carcinoma | | Growth Properties: | monolayer | | Description: | The cells are positive for keratin by immunoperoxidase staining. | | Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:2 to 1:4 is recommended | | Fluid Renewal: | 3 times weekly | | Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) | | Sterility: | Tests for mycoplasma (DAPI), bacteria and fungi were negative | | Isoenzymes: | G6PD, B | | Reverse Transcriptase: | negative | | Viruses: | SMRV negative, as confirmed by Real-Time PCR
| | Products: | keratin |
References:
Peterson WD Jr, Stulberg CS, Swanborg NK, Robinson AR. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968.
Peterson WD Jr, Stulberg CS, Simpson WF. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol.Med. 136: 1187, 1971
Van Wamel WJ, Vandenbroucke-Grauls CM, Verhoef J, Fluit AC. The effect of culture conditions on the in-vitro adherence of methicillin-resistant Staphylococcus aureus. J Med Microbiol. Aug;47(8): 705-9.1998.
Jane M. Gould , Jeffrey N. Weiser. The Inhibitory Effect of C-Reactive Protein on Bacterial Phosphorylcholine Platelet-Activating Factor Receptor-Mediated Adherence Is Blocked by Surfactant. The Journal of Infections Diseases 186: 361-371, 2002.
Adam J. Ratner, Elena S. Lysenko, Marina N, Paul, and Jeffrey N. Weiser. Synergistic proinflammatory responses induced by polymicrobial colonization of epithelial surfaces. Proc Natl Acad Sci USA 102(9): 3429-3434, 2005.
Romero-Steiner S, Caba J, Rajam G, Langley T, Floyd A, Johnson SE, Sampson JS, Carlone GM, Ades E. Adherence of recombinant pneumococcal surface adhesin A (rPsaA)-coated particles to human nasopharyngeal epithelial cells for the evaluation of anti-PsaA functional antibodies. Vaccine. 2006 Feb 2. |
| COLO-60H |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011. |
