来宝网Logo

热门词:生物显微镜 水质分析仪 微波消解 荧光定量PCR 电化学工作站 生物安全柜

AED
现在位置首页>医疗器械>体外诊断试剂>分子诊断试剂>CLS-117, CLS-117 Humane Struma Sarkom Zell-Linie 300329 (330329)
CLS-117,  CLS-117   Humane Struma Sarkom Zell-Linie    300329  (330329)
CLS-117,  CLS-117   Humane Struma Sarkom Zell-Linie    300329  (330329)
  • CLS-117,  CLS-117   Humane Struma Sarkom Zell-Linie    300329  (330329)

CLS-117, CLS-117 Humane Struma Sarkom Zell-Linie 300329 (330329)

产品报价:询价

更新时间:2014/8/28 10:33:50

地:美国

牌:cell-lines-service

号:CLS-117

厂商性质: 生产型,贸易型,服务型,

公司名称: 北京百杰生物科技有限公司

产品关键词:

313
访问人数
0
累计评论


李胜亮 : (13466675923) (13466675923)

(联系我时,请说明是在来宝网上看到的,谢谢!)


Designation:CLS-117
Depositor:CLS
Organism:human
Age/Stage:47-year old female
Tissue:sarcoma, thyroid, polymorph cells
Morphology:fibroblast
Growth Properties:monolayer
Description:in vitro established from the primary sarcoma of the thyroid gland of a 47-year-old female.
Culture Medium:RPMI 1640 medium supplemented with 2mm L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio:A ratio of 1:4 to 1:8 is recommended
Fluid Renewal:every 3 to 5 days
Freeze Medium:CM-1 (CLS order number 800125 or 800150)
Sterility:Tests for mycoplasma, bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:yes, in nude mice
DNA Profile (STR):Amelogenin: X
CSF1PO: 13
D13S317: 12
D16S539: 11
D5S818: 11
D7S820:11
THO1: 6
TPOX: 8
vWA: 14
D3S1358: 18
D21S11: 30
D18S51: 11
Penta E: 18
Penta D: 10
D8S1179: 15
FGA: 22.
CLS ? Cell Lines Service, 2011
Viruses:SMRV negative, as confirmed by Real-Time PCR


References:

J.Cancer Res. Clin. Oncol. 115 (suppl.), 1989


COLO-60H
Depositor:CLS
Organism:Homo sapiens (human)
Age/Stage:73 years of age
Gender:Male
Tissue:Colon transversum adenocarcinoma.
Morphology:Epithelial
Celltype:Colon adenocarcinoma
Culture Medium:DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400).
Subculturing:Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Fluid Renewal:Every 2 to 3 days
Freeze Medium:CM-1 (CLS order number 800125 or 800150)
Sterility:Tests for bacteria and fungi were negative
Biosafety Level:1
Tumorigenic:not tested
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011.