产品报价：询价
更新时间：2014/8/28 8:53:49
产地：美国
品牌：cell-lines-service
型号：CLS-117
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
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| COLO 205 | ||||||||||||||||||||||||||||||||||||||
| Depositor: | Moore | ||||||||||||||||||||||||||||||||||||||
| Organism: | Homo sapiens (human) | ||||||||||||||||||||||||||||||||||||||
| Ethnicity: | Caucasian | ||||||||||||||||||||||||||||||||||||||
| Age/Stage: | 70 years old | ||||||||||||||||||||||||||||||||||||||
| Gender: | male | ||||||||||||||||||||||||||||||||||||||
| Tissue: | Colon (metastatic site: ascites) | ||||||||||||||||||||||||||||||||||||||
| Morphology: | epithelial | ||||||||||||||||||||||||||||||||||||||
| Celltype: | Colorectal adenocarcinoma; Dukes' type D | ||||||||||||||||||||||||||||||||||||||
| Growth Properties: | cells grow loosely attached and in suspension | ||||||||||||||||||||||||||||||||||||||
| Description: | The cells are CSAp negative (CSAp-).The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. | ||||||||||||||||||||||||||||||||||||||
| Culture Medium: | RPMI 1640 medium supplemented with 4.5 g/L glucose, L-glutamine and 10% fetal bovine serum; cells grow loosely attached (MG-70, CLS order number 820700). In DMEM:Ham’s F12 (1:1 mixture) medium supplemented with L-glutamine and 5% fetal bovine serum, the cells will grow adherently (MG-40, CLS order number 820400). | ||||||||||||||||||||||||||||||||||||||
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 5 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | ||||||||||||||||||||||||||||||||||||||
| Split Ratio: | Subcultivation ratios of 1:2 to 1:10 are possible when all cells are pooled (suspended cells plus cells recovered using trypsin) | ||||||||||||||||||||||||||||||||||||||
| Fluid Renewal: | every 2 to 3 days | ||||||||||||||||||||||||||||||||||||||
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) | ||||||||||||||||||||||||||||||||||||||
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative | ||||||||||||||||||||||||||||||||||||||
| Biosafety Level: | 1 | ||||||||||||||||||||||||||||||||||||||
| Tumorigenic: | yes, in nude mice | ||||||||||||||||||||||||||||||||||||||
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 D3S1358: 16 D21S11: 30.2,33.2 D18S51: 18 Penta E: 13,15 Penta D: 9,11 D8S1179: 9,14 FGA: 21,23 CLS Cell Lines Service, 2010. | ||||||||||||||||||||||||||||||||||||||
| Isoenzymes: | G6PD, B; PGM1, 1-2; PGM3, 1-2; 6PGD, A; ES-D, 1-2, PEP-D, 1 | ||||||||||||||||||||||||||||||||||||||
| Reverse Transcriptase: | negative | ||||||||||||||||||||||||||||||||||||||
| Viruses: | SMRV negative, as confirmed by Real-Time PCR | ||||||||||||||||||||||||||||||||||||||
| Products: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/106 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) | ||||||||||||||||||||||||||||||||||||||
References: Semple TU et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. Gastl GA et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J.Cancer 55: 96-101, 1993. Bjork P et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J.Biol. Chem. 268: 24232-24241, 1993. | |||||||||||||||||||||||||||||||||||||||
