| Designation: | CCF-STTG1 | | Organism: | Homo sapiens (human) | | Ethnicity: | Caucasian | | Age/Stage: | 68 years | | Gender: | Female | | Tissue: | Brain | | Morphology: | astrocytoma, grade IV | | Celltype: | astrocytic | | Culture Medium: | RPMI 1640 supplemented with 4.5g/L glucose, 2 mM glutamine and 10% fetal bovine serum (MG-72, CLS order number 820702). | | Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. | | Split Ratio: | A ratio of 1:3 to 1:8 is recommended | | Fluid Renewal: | Every 2 to 3 days. | | Freeze Medium: | CM-1 (CLS order number 800125 or 800150) | | Sterility: | Tests for mycoplasma, bacteria and fungi were negative | | DNA Profile (STR): | Amelogenin: X, X
CSF1PO: 12
D13S317: 11, 13
D16S539: 11, 12
D5S818: 12, 13
D7S820: 10, 11
THO1: 7, 8
TPOX: 8, 11
vWA: 17
D3S1358: 16, 17
D21S11: 28, 29
D18S51: 15, 15
Penta E: 10, 10
Penta D: 11, 13
D8S1179: 13, 14
FGA: 20, 22
CLS ? Cell Lines Service, 2010. | | Antigen Expression: | HLA DR (on approx. 25% of cells) |
References:
In Vitro 19; 275,1983.
Fed.Proc. 43: 781, 1984.
Barna BP, Chou SM, Jacobs B, Ransohoff RM, Hahn JF, Bay JW. Enhanced DNA synthesis of human glial cell exposed to human leukocyte products. J Neuroimmunol 10(2): 151-158, 1985.
Krul ES, Tank J. Secretion of apolipoprotein E by an astrocytoma cell line. J Neurosci Res 32(2): 227-238, 1992.
Beaman L, Beaman BL. Differences in the interactions of Nocardia asteroides with macrophage, endothelial, and astrocytoma cell lines. Infect Immun 62(5): 1787-1798, 1994.
Thilakawardhana S, Everett DM, Murdock PR, Dingwall C, Owen JS. Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction. Neurobiol Aging 26(6): 813-823, 2005. |
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