产品报价：询价
更新时间：2014/8/22 17:30:17
产地：美国
品牌：cell-lines-service
型号：T406
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | T406 |
| Depositor: | Lichtenthaler |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Brain |
| Morphology: | fibroblast |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine, and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X, X CSF1PO: 12, 14 D13S317: 9, 9 D16S539: 11, 11 D5S818: 10, 13 D7S820: 10, 12 THO1: 7, 7 TPOX: 11, 11 vWA: 17, 17 D3S1358: 14, 16 D21S11: 28, 30 D18S51: 13, 18 Penta E: 7, 10 Penta D: 11, 11 D8S1179: 14, 14 FGA: 23, 26 CLS ? Cell Lines Service, 2010. |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Henn W et al. Polysomy of chromosome 7 is correlated with overexpression of the erbB oncogene in human glioblastoma cell line. Hum Genet 74: 104-6, 1986. Malter M et al. Natural cytotoxic cells from rat liver and spleen kill human glioma cells. J Cancer Res Clin Oncol 113: 498-501, 1987. Todorov DK et al. Antiproliferative activity of the non-myelotoxic antitumor agent of plant origin, Thaliblastine, on two human glioma cell lines. J Cancer Res Clin Oncol 118: 83-6, 1992. | |
