产品报价：询价
更新时间：2014/8/22 16:13:53
产地：美国
品牌：cell-lines-service
型号：NCH421K
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）BITTE BEACHTEN
Für den Erwerb der NCH421K Zelllinie müssen bestimmte Voraussetzungen erfüllt sein:
Nur gemeinnützige Institutionen k?nnen diese Zelllinien erwerben.
Ein MTA muss unterzeichnet werden.
Ohne ein unterzeichnetes Bestellformular der CLS Cell Lines Service GmbH kann kein Versand erfolgen.
| Designation: | NCH421K |
| Depositor: | C. Herold-Mende |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 66 years of age |
| Gender: | Male |
| Tissue: | Brain |
| Morphology: | Spheroid culture |
| Celltype: | Glioma / Glioblastoma WHO IV |
| Growth Properties: | Cluster in suspension |
| Description: | The NCH421K cell line was established by Christel Herold-Mende in 2008. NCH421K are highly CD133 positive. |
| Culture Medium: | Chemically defined serum-free media. CLS order no. 820403. |
| Subculturing: | Mechanical dissociation: Pipette 5-10 times using an Eppendorf pipette and 1000μl tips after centrifuging the cells 10min at 300xg at +20°C. |
| Split Ratio: | An initial ratio of 1:2 to 1:5 is recommended according to the growth rate. |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-2, serumfree Cryoprotective medium (CLS order no. 800225 or 800250) |
| Sterility: | Test for mycoplasma: PCR tested negative. Test for bacteria: PCR tested negative. |
| Biosafety Level: | 1 |
| Tumorigenic: | Yes |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,11 D13S317: 8,11 D16S539: 10,11 D5S818: 11,13 D7S820: 10,12 THO1: 6 TPOX: 8,11 vWA: 17,18 D3S1358: 14,16 D21S11: 30 D18S51: 13 Penta E: 7,12 Penta D: 9,13 D8S1179: 12,15 FGA: 21,25 CLS Cell Lines Service GmbH, 2012 |
References: Campos B. et al. Differentiation Therapy Exerts Antitumor Effexts on Stem-like Glioma Cells. Clin Cancer Res. 16(10): 2715--28, 2010. Ernst A., Homann S. Ahmadi R. et al. Genomic and Expression Profiling of Glioblastoma Stem Cell-Like Spheroid Cultures Identifies Novel Tumor-Relevant Genes Associated with Survival. Clin Cancer Res. 15: 6541-6550, 2009. | |
