产品报价：询价
更新时间：2014/8/22 16:11:08
产地：美国
品牌：cell-lines-service
型号：IMR-32
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | IMR-32 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 13 months |
| Gender: | male |
| Tissue: | brain (neuroblastoma) |
| Morphology: | fibroblast |
| Celltype: | Neuroblast, fibroblast |
| Growth Properties: | monolayer |
| Description: | There are two cell types present. A small neuroblast- like cell is predominant, and the other one is a large hyaline fibroblast. This cell line can be propagated to >80 serial subcultures. |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:6 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| DNA Profile (STR): | Amelogenin: X/Y CSF1PO: 11/12 D13S317: 9/9 D16S539: 8/10 D5S818: 11/12 D7S820: 9/10 THO1: 7/9,3 TPOX: 11/11 vWA: 15/15 D3S1358: 16/16 D21S11: 30/31 D18S51: 12/15 Penta E: 7/15 Penta D: 11/12 D8S1179: 13/13 FGA: 21/24 CLS ? Cell Lines Service, 2009. |
| Isoenzymes: | G6PD, B |
| Reverse Transcriptase: | negative |
| Virus Resistance: | echovirus 11 |
| Virus Susceptibility: | vesicular stomatitis (Indiana); herpes simplex; vaccinia; coxsackievirus B3; poliovirus 3 (poorly) |
References: Tumilowicz JJ et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res 30: 2110-8, 1970. Maestrini E et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc Natl Acad Sci USA 93: 674-8, 1996. Rostomily RC et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res 57: 3526-31, 1997. Mangoura d et al. 12-O-tetradecanoyl-phorbol-13-acetate-dependent up-regulation of dopaminergic gene expression requires Ras and neurofibromin in human IMR-32 neuroblastoma. J Neurochem 97: 97-103, 2006. Malisauskas M et al. Intermediate amylid oligomers of lysozyme: Is their cytotoxicity a particular case or general rule for amyloid? Biochemistry (Mosc) 71: 505-12, 2006. Kaur M et al. A tuber lectin from Arisaema jacquemontii Blume with anti-insect and anti-proliferatve properties. J Biochem Mol Biol 39: 432-40, 2006. | |
