产品报价：询价
更新时间：2014/8/22 15:46:59
产地：美国
品牌：cell-lines-service
型号：HBL-52
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HBL-52 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 47 years old |
| Gender: | female |
| Tissue: | Brain |
| Morphology: | epithelial |
| Celltype: | Meningioma |
| Growth Properties: | monolayer |
| Description: | The cell line was originally taken from a transitional meningioma grade I localized at the optic canal. |
| Culture Medium: | Mc Coy’s 5A medium supplemented with 2mM glutamine and 10% fetal bovine serum (MG-200, CLS order number 821200). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X, X CSF1PO: 10, 13 D13S317: 11, 12 D16S539: 11, 13 D5S818: 12, 13 D7S820: 10, 11 THO1: 6, 9.3 TPOX: 8, 8 vWA: 16, 20 D3S1358: 15, 15 D21S11: 30, 31 D18S51: 15, 16 Penta E: 11, 12 Penta D: 9, 10 D8S1179: 13, 13 FGA: 23, 26 CLS ? Cell Lines Service, 2010. |
References: Akat K., Mennel H.-D., Kremer P., Gassler N., Bleck C.K.E. and Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106: 337-347, 2003. | |
