产品报价：询价
更新时间：2014/8/22 15:24:42
产地：美国
品牌：cell-lines-service
型号：A-172
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | A-172 |
| Depositor: | Giard |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 53 years |
| Gender: | male |
| Tissue: | brain |
| Celltype: | glioblastoma |
| Growth Properties: | monolayer |
| Description: | The A-172 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | DMEM : Ham’s F12 medium (1:1 mixture) supplemented with L-glutamine and 5% FBS (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 9,12 D13S317: 11 D16S539: 12 D5S818: 11,12 D7S820: 11 THO1: 6,9.3 TPOX: 8,11 vWA: 20 D3S1358: 14,18 D21S11: 28,32.2 D18S51: 12,13 Penta E: 5,10 Penta D: 9,13 D8S1179: 13,14 FGA: 20,22 CLS ? Cell Lines Service, 2010. |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Pinkerton H and Rana MW. Cytotoxic effects of 1,3-bis(2 chloroethyl)-1-nitrosourea (BCNU) on cultured human glioblastomas. Proc Soc Exp Biol Med 151:532-4, 1976. Olopade OI et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res 52: 2523-9, 1992. Debinski W et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J Biol Chem 271: 22428-33, 1996. Cordes N et al. beta1-integrin-mediated signaling essentially contributes to cell survival after radiation-induced genotoxic injury. Oncogene 25: 1378-90, 2006. Ohnishi K et al. Induction of radiation resistance by a heat shock protein inhibitor, KNK437, in human glioblastoma cells. Int J Radiat Biol 82: 569-75, 2006. Shirato K et al. Phenoxazine derivatives induce caspase-independent cell death in human glioblastoma cell lines, A-172 and U-251 MG. Oncol Rep 17: 201-8, 2007. | |
