产品报价：询价
更新时间：2014/8/22 14:45:03
产地：美国
品牌：cell-lines-service
型号：SK-MEL-1
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | SK-MEL-1 |
| Depositor: | Old |
| Organism: | Homo sapiens (human) |
| Ethnicity: | caucasian |
| Age/Stage: | 29 years |
| Gender: | male |
| Tissue: | melanoma, malignant; skin; from metastatic site: lymphatic system |
| Morphology: | spherical |
| Growth Properties: | suspension |
| Description: | F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation. |
| Culture Medium: | Minimum essential medium Eagle (Earle's BSS) supplemented with 2 mM L-glutamine, 0.1 mM non-essential amino acids (NEAA), 1.0 mM sodium pyruvate, and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 x 105 cells/ml and maintain at 2-5 x 105 cells/ml. |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-5 (CLS order number: 800525, 25ml, 800550, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms pigmented malignant melanomas; also forms tumors in the cheek pouch of cortisone treated hamsters |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 10,13 D7S820: 8,11 THO1: 7,9 TPOX: 9 vWA: 14,17 D3S1358: 16 D21S11: 29,31 D18S51: 13,15 Penta E: 13,21 Penta D: 13,14 D8S1179: 13,14 FGA: 17 CLS ? Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type A; Rh+ |
| Isoenzymes: | PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; |
| Products: | melanin |
References: Oettgen HF et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J Natl Cancer Inst 41: 827-43, 1968. Giraldo G et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J Exp Med 133: 454-78, 1971. Carey TE et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc Natl Acad Sci USA 73: 3278-82, 1976. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Geiger T et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des 13: 35-45, 1998. Baker DP et al. N-terminally PEGylated human interferon-beta-1a with improved pharmacokinetic properties and in vivo efficacy in a melanoma angiogenesis model. Bioconjug Chem 17: 179-88, 2006. Leon F et al. Isolation, structure elucidation, total synthesis, and evaluation of new natural and synthetic ceramides on human SK-MEL-1 melanoma cells. J Med Chem 49: 5830-9, 2006. | |
