产品报价：询价
更新时间：2014/8/22 14:20:33
产地：美国
品牌：cell-lines-service
型号：MEL-CLS-1
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | MEL-CLS-1 (FO-1) |
| Depositor: | CLS, H. L?hrke |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Melanoblastoma |
| Growth Properties: | monolayer |
| Description: | in vitro etablished from the primary Melanoblastoma by CLS. According to our sequencing and WB results this cell line carries a BRAF V600E mutation. |
| Culture Medium: | DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine, and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 is recommended |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice (Virales Profil: Sendai, Ektromelie, Polyoma, K-Virus, Kilham, Reo 3, PVM, LCM, M.pulmonis, MVM, Theiler's GD VII, toolan's H-1, MHV, LDV, RCV/SDA, M-Adenovirus, B.piliformis: negativ) |
| Karyotype: | Modalzahl 51; Rang 38-56 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,12 D13S317: 12 D16S539: 9,12 D5S818: 12,13 D7S820: 9,11 THO1: 9 TPOX: 8 vWA: 17,18 D3S1358: 15,18 D21S11: 27 D18S51: 17 Penta E: 14,17 Penta D: 9 D8S1179: 12,14 FGA: 19,23 CLS ? Cell Lines Service, 2010. |
| DNA-Index: | 1,6 Cellcyklus day 4: G1=89,5%, S=5,25%, G2+M=5,25% |
References: Not published | |
