产品报价：询价
更新时间：2014/8/22 14:18:30
产地：美国
品牌：cell-lines-service
型号：IGR-1
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | IGR-1 |
| Depositor: | Rot |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 42 years |
| Gender: | male |
| Tissue: | skin |
| Morphology: | polygonal |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | The IGR-1 cell line has been established from the metastatic melanoma in a growing lymph node. According to our sequencing results this cell line carries a BRAF V600K mutation. |
| Culture Medium: | DMEM supplemented with 4.5 g/L glucose, 2 mM L-glutamine, and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 13 D16S539: 11,13 D5S818: 10,11 D7S820: 10,11 THO1: 7,9.3 TPOX: 8 vWA: 17,18 D3S1358: 14,17 D21S11: 32.2 D18S51: 16 Penta E: 7,11 Penta D: 10 D8S1179: 10 FGA: 23,24 CLS ? Cell Lines Service, 2010. |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Aubert C et al. [Differenttiation and tumorigenicity of human malignant melanocyes. Comparative study of two “non pigmented” and pigmented lines from the same fibroblastoid cells]. CR Seances Acad Sci D 288: 919-22, 1979. Aubert C et al. Tumorigenicity of human malignant melanocytes in nude mice in relation to their differentiation in vitro. J Natl Cancer Inst 64: 1029-40, 1980. Aubert C et al. Differentiation and tumorgenicity of human malignant melanocytes in relation to their culture conditions. J Natl Cancer Inst 72: 3-12, 1984. Laube F. Co-localization oc CD44 and urokinase-type plasminogen activator on the surface of human melanoma cells. Anticancer Res 20: 5045-8, 2000. Bolling B et al. VEGF production, cell proliferation and apoptosis of human IGR 1 melanoma cells under nIFN-alpha/beta and rIFN-gamma treatment. Exp Dermatol 9: 327-35, 2000. Berggren P et al. Detecting homozygous deletions in the CDKN2A(p16(INK4a))/ARF(p14(ARF)) gene in urinary bladder cancer using real-time quantitative PCR. Clin Cancer Res 9: 235-42, 2003. | |
