产品报价：询价
更新时间：2014/8/22 14:35:25
产地：美国
品牌：
型号：MML-1
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | MML-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Tissue: | skin |
| Morphology: | epithelial |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation. |
| Culture Medium: | RPMI 1640 supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10 D13S317: 8,13 D16S539: 10,11 D5S818: 10,12 D7S820: 10,12 THO1: 6,10 TPOX: 11 vWA: 17,18 D3S1358: 17 D21S11: 31 D18S51: 13,14 Penta E: 7,11 Penta D: 14 D8S1179: 13,14 FGA: 23 CLS ? Cell Lines Service, 2010. |
| Reverse Transcriptase: | negative |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Concepcio′n de Ine′s, Bjoern Cochlovius, Stefanie Schmidt, Sergey Kipriyanov, Hans-Juergen Rode, and Melvyn Little. Apoptosis of a Human Melanoma Cell Line Specifically Induced by Membrane-Bound Single-Chain Antibodies1. The Journal of Immunology, 163: 3948–3956, 1999. | |
