产品报价：询价
更新时间：2014/8/22 13:56:20
产地：美国
品牌：cell-lines-service
型号：Colo-38
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | Colo-38 |
| Depositor: | Fisher |
| Organism: | Homo sapiens (human) |
| Tissue: | Melanoma |
| Growth Properties: | adherent |
| Description: | Authentication by STR analysis has revealed that the cell line Colo-38 has very simular data as compared to the cell lines M4A4 and MDA-MB-435s. Thus, Colo-38 may be a derivative of one of these cell lines or cross-contaminated. |
| Culture Medium: | RPMI 1640 supplemented with glutamine and 10% fetal bovine serum. |
| Subculturing: | Remove medium and rinse using PBS. Add Accutase solution and incubate at 37°C for 10 minutes. As soon as all the cells have come off of the button of the cell culture flask, combine the single cell solution and dispense into new flasks. |
| Split Ratio: | A ratio of 1:5 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1, Cell Lines Service |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in BALB/c nu/nu mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11 D13S317: 12 D16S539: 9,13 D5S818: 11,12 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 16,18 D3S1358: 14,16 D21S11: 30 D18S51: 13,17 Penta E: 10,12 Penta D: 9,11 D8S1179: 13 FGA: 21 CLS ? Cell Lines Service, 2010. |
References: Ferrone S, Pellegrino MA. Cytotoxic antibodies to cultured melanoma cells in the sera of melanoma patients. J Natl Cancer Inst. 58(5):1201-4, 1977. Giacomini P, Aguzzi A, Tecce R, Fisher PB, Ferrone S. A third polypeptide associated with heavy and light chain subunits of class I HLA antigens in immune interferon-treated human melanoma cells. Eur J Immunol. 15(9):946-51, 1985. Deichmann M, Huder JB, Kleist C, Naher H, Schupbach J, Boni J. Detection of reverse transcriptase activity in human melanoma cell lines and identification of a murine leukemia virus contaminant. Arch Dermatol Res. 296(8):345-52, 2005. Tilgen W, Matzku S, Kaufmann I, Engstner M, Brüggen J, Dippold W, Petzoldt D. Modulation of melanoma-associated antigens by monoclonal antibodies as visualized by radioimmunoelectron microscopy and radioantibody binding assay. Arch Dermatol Res. 279 Suppl:S116-26, 1987. Mohler T, Scheibenbogen C, Hafele J, Willhauck M, Keilholz U. Regulation of interleukin-8 mRNA expression and protein secretion in a melanoma cell line by tumour necrosis factor-alpha and interferon-gamma. Melanoma Res. 6(4):307-11, 1996. | |
