产品报价：询价
更新时间：2014/8/22 14:10:28
产地：美国
品牌：cell-lines-service
型号：HS-695T
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HS-695T |
| ATCC Number: | HTB-137 |
| Depositor: | R.B. Owens |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 26 years |
| Gender: | male |
| Tissue: | melanoma, amelanotic; from metastatic site: lymph node |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | HS-695T was isolated in 1973 from the lymph node metastasis of an amelanotic melanoma by A. Creasey et al. The cells had a doubling time of approximately 48 hours at passages 19 through 40 and grew moderately in semi-solid medium. According to our sequencing and WB results this cell line carries a BRAF V600E mutation. |
| Culture Medium: | DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immunosuppressed mice |
| Karyotype: | (P19-40) mode = 52; Y chromosome present |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 12 D16S539: 9,13 D5S818: 9 D7S820: 9,10 THO1: 6 TPOX: 8 vWA: 18 D3S1358: 15 D21S11: 29 D18S51: 18 Penta E: 18,5 Penta D: 9/12,9/12 D8S1179: 13,15 FGA: 21,24 CLS ? Cell Lines Service, 2010. |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 0; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0427 |
References: Creasey AA et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. | |
