产品报价：询价
更新时间：2014/8/22 13:53:49
产地：美国
品牌：cell-lines-service
型号：A-431
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | A-431 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 85 years old |
| Gender: | female |
| Tissue: | Skin |
| Morphology: | Epithelial, flat polygonal |
| Celltype: | epidermoid (squamous cell) carcinoma |
| Growth Properties: | monolayer |
| Description: | The A-431 cell line was established by D.J. Giard in 1973. |
| Culture Medium: | DMEM supplemented with 4.5 g/L glucose, 2 mM L-glutamine and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10-15 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in immunosuppressed mice |
| Genotype: | Wild type (with respect to BRAFmutation analysis) |
| Karyotype: | Six marker chromosomes with rearrangements: der(6), der(7), der(17), der(21), dic(13;14), and dic(14;18). Amplification of the C-MYC oncogene at 8q24 in two marker chromosomes: dup(8)(q24) and der(15)t(8;15)(q22;p11). |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 9,13 D16S539: 12,14 D5S818: 12,13 D7S820: 10 THO1: 9 TPOX: 11 vWA: 15,17 D3S1358: 14 D21S11: 28,30 D18S51: 13,17 Penta E: 12,13 Penta D: 9,11 D8S1179: 13 FGA: 20 CLS ? Cell Lines Service, 2010. |
| Receptors Expressed: | EGF-binding sites |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 0; AK-1, 1; GLO-1, 2 |
| Products: | HBp17 |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Haigler H et al. Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431. Proc Natl Acad Sci USA 75: 3317-21, 1978. Kovelman R et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J Virol 70: 7549-60, 1996. Lee JH et al. The proximal promoter of the human transglutaminase 3 gene. J Biol Chem 271: 4561-8, 1996. Chang K and Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc Natl Acad Sci USA 93: 136-40, 1996. Wizemann TM et al. Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens. Proc Natl Acad Sci USA 93: 7985-90, 1996. Pedrazzini E et al. Genomic rearragements involving rDNA and centromeric heterochromatin in vulvar epidermoid carcinoma cell line A-431. Cancer Genetics and Cytogenetics 143(1): 50-58, 2003. Katso RM et al. Phosphoinositide 3-Kinase C2beta regulates cytoskeletal organization and cell migration via Rac-dependent mechanisms. Mol Biol Cell 17: 3729-44, 2006. Cherepanova OA et al. Contribution of alpha2beta1, alpha3beta1, alpha6beta4 integrins and 67 kDa laminin receptor to the interaction of epidermoid carcinoma A-431 cells with laminin-2/4. Cell Biol Int 30: 784-92, 2006. Sohn K et al. An in vitro assay to study the transcriptional response during adherence of Canadia albicans to different human epithelia. FEMS Yeast Res 6: 1085-93, 2006. | |
