产品报价：询价
更新时间：2014/8/22 13:51:30
产地：美国
品牌：cell-lines-service
型号：A-375
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | A-375 |
| Depositor: | Cell Lines Service |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 54 years old |
| Gender: | female |
| Tissue: | skin |
| Celltype: | malignant melanoma |
| Growth Properties: | monolayer |
| Description: | The A-375 cell line was established by D.J. Giard in 1973. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation. |
| Culture Medium: | DMEM supplemented with 4.5 g/L glucose, 2 mM L-glutamine, and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17 D3S1358: 15,17 D21S11: 29,30 D18S51: 12,17 Penta E: 10,12 Penta D: 9,15 D8S1179: 11,14 FGA: 23 CLS ? Cell Lines Service, 2010. |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Giard DJ et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst 51: 1417-23, 1973. Gershwin ME et al. Immunobiology of heterotransplanted human tumors in nude mice. J Natl Cancer Inst 58: 1455-63, 1977. Sharma SD et al. Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors. Proc Natl Acad Sci USA 93: 13715-20, 1996. Cormier JN et al. Heterogeneous expression of melanoma-associated antigens and HLA-A2 in metastatic melanoma in vivo. Int J Cancer 75: 517-24, 1998. Fecker LF et al. Selective induction of apoptosis in melanoma cells by tyrosinase promoter-controlled CD95 ligand overexpression. J Invest Dermatol 124: 221-8, 2005. Deichmann M et al. Detection of reverse transcriptaseactivity inhuman melanoma cell lines and identification of a murine leukemia virus contaminant. Arch Dermatol Res 296: 345-52, 2005. Mahdian R et al. Dendritic cells, pulsed with lysate of allogeneic tumor cells, are capable of stimulating MHC-restricted antigen-specific antitumor T cells. Med Oncol 23: 273-82, 2006. | |
