产品报价：询价
更新时间：2014/8/22 12:17:07
产地：美国
品牌：cell-lines-service
型号：T24
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | T24 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 81 years |
| Gender: | female |
| Tissue: | bladder, urinary |
| Morphology: | epithelial |
| Celltype: | transitional cell carcinoma |
| Growth Properties: | monolayer |
| Description: | Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines. T24 cells have a generation time of 19 hours. |
| Culture Medium: | DMEM:Ham’s F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine, 15 mM Hepes and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 5-10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:3 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in hamster cheek pouch; not in nude mice |
| Oncogene: | H-ras+ |
| Karyotype: | hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/12 D13S317: 12/12 D16S539: 9/9 D5S818: 10/12 D7S820: 10/11 THO1: 6/6 TPOX: 8/11 vWA: 17/19 D3S1358: 16/21 D21S11: 29/29 D18S51: 16/18 Penta E: 7/10 Penta D: 11/15 D8S1179: 9/14 FGA: 17/22 CLS ? Cell Lines Service, 2009. |
| Antigen Expression: | HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4 |
| Isoenzymes: | Me-2, 1-2; PGM3, 1; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1; G6PD, B; Phenotype Frequency Product: 0.0216 |
| Products: | tumor specific antigen |
References: Bubenik J et al. Cellular and humoral immune responses to human urinary bladder carcinomas. Int J Cancer 5: 310-9, 1970. Bubenik J et al. Cellular immunity to renal carcinomas in man. Int J Cancer 8: 503-513, 1971. O'Toole C et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int J Cancer 10: 77-91, 1972. Bubenick J et al. Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int J Cancer 11: 765-73, 1973. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One-hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res 57: 516-23, 1997. Bender CM et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res 58: 95-101, 1998. Shen SS et al. Expression of estrogen receptors-alpha and -beta in bladder cancer cell lines and human bladder tumor tissue. Cancer 106: 2610-6, 2006. Roomi MW et al. Antitumor effect of ascorbic acid, lysine, proline, arginine, and green tea extract on bladder cancer cell line T-24. Int J Urol 13: 415-9, 2006. | |
