产品报价：询价
更新时间：2014/8/22 11:54:23
产地：美国
品牌：cell-lines-service
型号：CLS-439
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | CLS-439 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | European |
| Age/Stage: | 61 years |
| Gender: | male |
| Tissue: | carcinoma; bladder, urinary |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | established from the primary bladder carcinoma of a 61-year-old male, 1998 |
| Culture Medium: | Mc Coy’s 5A medium supplemented with 2mM glutamine and 10% fetal bovine serum (MG-200, CLS order number 821200). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress(1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 10,13 D5S818: 11 D7S820:10,11 THO1: 7 TPOX: ,9,10 vWA: 17 D3S1358: 16 D21S11: 29,31 D18S51: 14 Penta E: 12,16 Penta D: 9,12 D8S1179: 11,13 FGA: 20. CLS ? Cell Lines Service, 2011 |
References: not published | |
