产品报价：询价
更新时间：2014/8/22 12:13:07
产地：美国
品牌：cell-lines-service
型号：RT4
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | RT4 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 63 years |
| Gender: | male |
| Tissue: | transitional cell papilloma; bladder, urinary |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | McCoy's 5a medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-200, CLS order number 821200). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:4 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in cheek pouch of steroid treated hamsters |
| Karyotype: | (P174) hyperdiploid and hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, translocations and minutes |
| Antigen Expression: | HLA A25(10), A3, B12, Cw3; Blood Type O |
| Isoenzymes: | Me-2, 1; PGM1, 1-2; PGM3, 1-2; ES-D, 1-2; AK-1, 1; GLO-1, 1-2; G6PD, B; Phenotype Frequency Product: 0.0050 |
References: Rigby CC et al. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br J Cancer 24: 746-54, 1970. O'Toole C et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int J Cancer 10: 77-91, 1972. Franks LM et al. Letter: HeLa cells and RT4 cells. Science 188: 168, 1975. Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst 58: 209-14, 1977. Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221-6, 1977. Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J Natl Cancer Inst 61: 967-78, 1978. Lasfargues EY et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-9, 1979. Pollack MS et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J Natl Cancer Inst 66: 1003-12, 1981. Perabo FG et al. Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of surviving in transitional cell cancer. Anticancer Res 26: 2129-35, 2006. Oka N et al. Role of phosphatidyllinositol-3 kinase/Akt pathway in bladder cancer cell apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand. Cancer Sci 97: 1093-8, 2006. | |
