产品报价：询价
更新时间：2014/8/22 12:06:42
产地：美国
品牌：cell-lines-service
型号：RT-112
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | RT-112 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Tissue: | Urinary bladder |
| Morphology: | Epithelial |
| Celltype: | Carcinoma |
| Growth Properties: | Adherent, monolayer |
| Culture Medium: | RPMI 1640 media supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | Yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,11 D13S317: 13,14 D16S539: 11,13 D5S818: 10,13 D7S820: 12,11 THO1: 7 TPOX: 8,11 vWA: 14,17 D3S1358: 15 D21S11: 27,30 D18S51: 15 Penta E: 12,16 Penta D: 10,11 D8S1179: 13,15 FGA: 23 CLS ? Cell Lines Service, 2010. |
| DNA-Index: | 2,1 |
| Antigen Expression: | Cytokeratine (4),5,(6), 7, 8, 13, 17, 18, 19, Desmoplakin. |
References: Benham F et al. Alkaline phosphatase activity in human bladder tumor cell lines. J Histochem Cytochem 25: 266-74, 1977. Marshall CL et al. Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas. J Nat Cancer Inst 58: 1743-51, 1977. Wilson PD et al. Immunoelectron microscopic localisation of keratin and luminal epithelial antigens in normal and neoplastic urothelium. Path Res Pract. 175: 289-298, 1982. Boxberger HJ et al. A new method for the 3-D in vitro growth of human RT112 bladder carcinoma cells using the alginate culture technique. Biol Cell 82: 109-19, 1994. Wang MR et al. Cytogenetic characterization of a bladder carcinoma cell line by fluorescence in situ hybridization. Cancer Genet Cytogenet 82: 170-4, 1995. Nista A et al. beta 1-Integrin expression and function in human bladder cancer cells: modulation by TNF alpha. Anticancer Res 16: 581-8, 1996. Wu Q et al. Growth arrest and suppression of tumorigenicity of bladder-carcinoma cell lines induced by the P16/CDKN2 (p16INK4A, MTS1) gene and other loci on human chromosome 9. Int J Cancer 65: 840-6, 1996. Chresta CM et al. Hypersensitivity of human testicular tumors to etoposide-induced apoptosis is associated with functional p53 and high Bax:Bcl-2 ratio. Cancer Res 56: 1834-41, 1996. Chau Y et al. Antitumor efficacy of a novel polymer-peptide-drug conjugate in human tumor xenograft models. Int J Cancer 118: 1519-26, 2006. Pellizzari L et al. PAX8 expression in human bladdercancer. Oncol Rep 16: 1015-20, 2006. | |
