产品报价：询价
更新时间：2014/8/22 11:46:34
产地：美国
品牌：cell-lines-service
型号：5637
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | 5637 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 68 years |
| Gender: | male |
| Tissue: | Bladder (urinary) |
| Morphology: | epithelial |
| Celltype: | carcinoma |
| Growth Properties: | monolayer |
| Description: | The 5637 cell line has been established from the primary bladder carcinoma (grade II) of a patient by Dr. G. Cannon in 1974. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700) |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 8-10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:5 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | in nude mice |
| Karyotype: | Phenotype Frequency Product: 0.0056 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 9 D5S818: 11,12 D7S820: 10,11 THO1: 7,9 TPOX: 8,9 vWA: 18,18 D3S1358: 15,17 D21S11: 36 D18S51: 16,18 Penta E: 10,12 Penta D: 11 D8S1179: 10,16 FGA: 22 CLS ? Cell Lines Service, 2010. |
| Isoenzymes: | Me-2, 1; PGM3, 2; PGM1, 1; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B; |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
| Products: | IL-1, IL-6, G-CFS, GM-CSF, SCF |
References: Fogh J et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in mice. J Natl Cancer Inst 59: 221-6, 1977. Fogh J. Cultivation, characterization and identification of human tumor cells with emphasis on kidney, testis and bladder tumors. Natl Cancer Inst Monogr 49: 5-9, 1978. Myers CD et al. A cell line secreting stimulating factors for CFU-GEMM culture. Blood 64(1): 152-155, 1984. Schnier JB et al. G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637. Proc Natl Acad Sci USA 93: 5941-6, 1996. Bellet D et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res 57: 516-23, 1997. Hu SX et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res 57: 3339-43, 1997. Bender CM et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res 58: 95-101, 1998. Baierlein SA et al. Combined effect of tumor necrosis factor-alpha and ionizing radiation on the induction of apoptosis in 5637 bladder carcinoma cells. Strahlenther Onkol 182: 467-72, 2006. Pellizzari L et al. PAX8 expression in human bladder cancer. Oncol Rep 16: 1015-20, 2006. Gazzaniga P et al. Gemcitabine-induced apoptosis in 5637 cell line: an in-vitro model for high-risk superficial bladder cancer. Anticancer Drugs 18: 179-185, 2007. | |
