产品报价：询价
更新时间：2014/8/22 12:04:30
产地：美国
品牌：cell-lines-service
型号：HB-CLS-2
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HB-CLS-2 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 50 years |
| Gender: | male |
| Tissue: | carcinoma GIII; bladder, urinary |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | etablished from the primary bladder carcinoma grading III of a 50-year-old male |
| Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress(1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 7,10 D13S317:11,12 D16S539:12 D5S818:10,12 D7S820: 8,9 TH01:8,10 TPOX:11 vWA:15,18 D3S1358: 16 D21S11: 32.2,35.2 D18S51: 15,17 Penta E: 13 Penta D: 11,13 D8S1179: 13 FGA: 21,23 CLS – Cell Lines Service 2011. |
References: not published | |
