产品报价：询价
更新时间：2014/8/22 11:56:38
产地：美国
品牌：cell-lines-service
型号：ECV-304
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | ECV-304 |
| Depositor: | Takahashi |
| Organism: | Homo sapiens (human) |
| Synonym(s): | Derivative of T24 |
| Tissue: | urinary bladder; carcinoma |
| Morphology: | epithelial |
| Growth Properties: | adherent |
| Description: | DNA profiling studies, performed by ATCC, revealed that STR patterns of the endothelial line ECV-304 and the human bladder line T24 were very similar, suggesting that ECV-304 was a derivative of T24. Furthermore, ATCC karyotypes of the two lines show two shared-marker chromosomes. Thus, ECV-304 mopst probably is a derivative of T24, a line that was developed a couple of years earlier. It is important to emphasize that all stocks of ECV show similar properties, not just those from ATCC. It is clear that the contamination took place before the cell banks independently received initial stocks. Independent laboratories have reported that ECV showed some biomarkers expected of endothelial cell lines, namely Factor VIII, tubule formation on Matrigel, and/or Weibel-Palade bodies. Studies to determine if T24 exhibits any such markers are underway. |
| Culture Medium: | Medium 199 supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-100, CLS order number 821100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:6 to 1:10 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in BALB/c nu/nu mice |
| DNA Profile (STR): | Amelogenin: X, X CSF1PO: 12 D13S317: 12 D16S539: 9 D5S818: 10 D7S820: 10, 11 TPOX: 8, 11 THO1:6 vWA: 17 D3S1358: 16, 16 D21S11: 29, 29 D18S51: 16, 18 Penta E: 7, 10 Penta D: 11, 15 D8S1179: 14, 14 FGA: 17, 22 CLS ? Cell Lines Service, 2010. |
References: Takahashi K et al. Spontaneous transformation and immortalization of human endothelial cells. In Vitro Cell. Dev. Biol. 26: 265-274, 1990. Takahashi K and Sawasaki Y. Human endothelial cell line, ECV304, produces pro-urokinase [letter]. In Vitro Cell. Dev. Biol. 27A: 766-768, 1991. Takahasi K and Sawasaki Y. Rare spontaneously transformed human endothelial cell line provides useful research tool [letter]. In Vitro Cell. Dev. Biol. 28A: 380-382, 1992. | |
