产品报价：询价
更新时间：2014/8/22 11:25:32
产地：美国
品牌：cell-lines-service
型号：NCH612
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）BITTE BEACHTEN
Für den Erwerb der NCH612 Zelllinie müssen bestimmte Voraussetzungen erfüllt sein:
Nur gemeinnützige Institutionen k?nnen diese Zelllinien erwerben.
Ein MTA muss unterzeichnet werden.
Ohne ein unterzeichnetes Bestellformular der CLS Cell Lines Service GmbH kann kein Versand erfolgen.
| Designation: | NCH612 |
| Depositor: | C. Herold-Mende |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 39 years of age |
| Gender: | Male |
| Tissue: | Brain |
| Morphology: | Spheroid culture |
| Celltype: | Glioma / Oligodendroglioma WHO °II, IDH1 mutant (R132H) |
| Growth Properties: | Cluster in suspension |
| Description: | The NCH612 cell line was established by Christel Herold-Mende in 2008. |
| Culture Medium: | Chemically-defined serum-free medium, ready-to-use (CLS order no. 820403) |
| Subculturing: | Mechanical dissociation: Pipette 5-10 times using an Eppendorf pipette and 1000μl tips after centrifuging the cells 10min at 300xg at +20°C. |
| Split Ratio: | An initial ratio of 1:2 to 1:5 is recommended according to the growth rate. |
| Fluid Renewal: | 2 times weekly |
| Freeze Medium: | CM-2, serumfree Cryoprotective medium (CLS order no. 800225 or 800250) |
| Sterility: | Tests for mycoplasma: PCR tested negative. Tests for bacteria: PCR tested negative. |
| Biosafety Level: | 1 |
| Tumorigenic: | Not determined. |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317:10 D16S539: 11,13 D5S818: 11,13 D7S820: 10,11 THO1: 6,7 TPOX: 8,12 vWA: 17 D3S1358: 14,18 D21S11: 28,31 D18S51: 13 Penta E: 11,14 Penta D: 9,12 D8S1179: 13 FGA: 21 CLS Cell Lines Service GmbH, 2012. |
