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CCF-STTG1， CCF-STTG1 Humane Astrozytom Zell-Linie 300388（330388）

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更新时间：2014/8/22 15:28:32

产地：美国

品牌：cell-lines-service

型号：CCF-STTG1

厂商性质：生产型,贸易型,服务型,

公司名称：北京百杰生物科技有限公司

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Designation:	CCF-STTG1
Organism:	Homo sapiens (human)
Ethnicity:	Caucasian
Age/Stage:	68 years
Gender:	Female
Tissue:	Brain
Morphology:	astrocytoma, grade IV
Celltype:	astrocytic
Culture Medium:	RPMI 1640 supplemented with 4.5g/L glucose, 2 mM glutamine and 10% fetal bovine serum (MG-72, CLS order number 820702).
Subculturing:	Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.
Split Ratio:	A ratio of 1:3 to 1:8 is recommended
Fluid Renewal:	Every 2 to 3 days.
Freeze Medium:	CM-1 (CLS order number 800125 or 800150)
Sterility:	Tests for mycoplasma, bacteria and fungi were negative
DNA Profile (STR):	Amelogenin: X, X CSF1PO: 12 D13S317: 11, 13 D16S539: 11, 12 D5S818: 12, 13 D7S820: 10, 11 THO1: 7, 8 TPOX: 8, 11 vWA: 17 D3S1358: 16, 17 D21S11: 28, 29 D18S51: 15, 15 Penta E: 10, 10 Penta D: 11, 13 D8S1179: 13, 14 FGA: 20, 22 CLS ? Cell Lines Service, 2010.
Antigen Expression:	HLA DR (on approx. 25% of cells)
References: In Vitro 19; 275,1983. Fed.Proc. 43: 781, 1984. Barna BP, Chou SM, Jacobs B, Ransohoff RM, Hahn JF, Bay JW. Enhanced DNA synthesis of human glial cell exposed to human leukocyte products. J Neuroimmunol 10(2): 151-158, 1985. Krul ES, Tank J. Secretion of apolipoprotein E by an astrocytoma cell line. J Neurosci Res 32(2): 227-238, 1992. Beaman L, Beaman BL. Differences in the interactions of Nocardia asteroides with macrophage, endothelial, and astrocytoma cell lines. Infect Immun 62(5): 1787-1798, 1994. Thilakawardhana S, Everett DM, Murdock PR, Dingwall C, Owen JS. Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction. Neurobiol Aging 26(6): 813-823, 2005.

COLO-60H

Depositor:

CLS

Organism:

Homo sapiens (human)

Age/Stage:

73 years of age

Gender:

Male

Tissue:

Colon transversum adenocarcinoma.

Morphology:

Epithelial

Celltype:

Colon adenocarcinoma

Culture Medium:

DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400).

Subculturing:

Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium.

Fluid Renewal:

Every 2 to 3 days

Freeze Medium:

CM-1 (CLS order number 800125 or 800150)

Sterility:

Tests for bacteria and fungi were negative

Biosafety Level:

Tumorigenic:

not tested

DNA Profile (STR):

Amelogenin: X,Y
CSF1PO: 11,15
D13S317: 11
D16S539: 9,13
D5S818: 9,16
D7S820: 7.3,10
THO1: 6,9.3
TPOX: 7,10
vWA: 15,16
D3S1358: 15
D21S11: 29,33.2
D18S51: 13,15
Penta E: 11,13
Penta D: 14
D8S1179: 11
FGA: 21,24
CLS ? Cell Lines Service, 2011.

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CCF-STTG1， CCF-STTG1 Humane Astrozytom Zell-Linie 300388（330388）

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