产品报价：询价
更新时间：2014/8/22 10:33:40
产地：美国
品牌：cell-lines-service
型号：CX-1
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | CX-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 44 years |
| Gender: | female |
| Tissue: | adenocarcinoma, colorectal; colon |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Culture Medium: | DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine and 10% fetal bovine serum (MG-30, CLS order number 820300). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 5-10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:8 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9 TPOX: 8,9 vWA: 17,19 D3S1358: 15,17 D21S11: 29,30 D18S51: 13 Penta E: 14,16 Penta D: 11,13 D8S1179: 10 FGA: 20,22 CLS ? Cell Lines Service, 2010. |
| Reverse Transcriptase: | negative |
| Viruses: | Test for SMR-Provirus: env-gene negative / gag-gene negative SMRV negative, as confirmed by Real-Time PCR |
| Products: | Cytokeratine 8, 18, 19 |
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