产品报价：询价
更新时间：2014/8/22 10:20:11
产地：美国
品牌：cell-lines-service
型号：COLO-94H
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | COLO-94H |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 70 years |
| Gender: | male |
| Tissue: | adenocarcinoma, colorectal; colon, ascendes |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | established from the primary adenocarcinoma of the colon of a 70 year-old male, Cell Lines Service |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. It is recommended to coat the tissue flasks with collagen prior to cell cultivation. |
| Split Ratio: | A ratio of 1:2 to 1:8 is recommended |
| Fluid Renewal: | 1 to 2 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,14 D13S317: 11 D16S539: 13 D5S818: 12 D7S820: 8 THO1: 7,9.3 TPOX: 8 vWA: 15,19 D3S1358: 15,17 D21S11: 27,28 D18S51: 18 Penta E: 17 Penta D: 12,13 D8S1179: 12 FGA: 21 CLS ? Cell Lines Service, 2011. |
| Reverse Transcriptase: | negative |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
| Products: | Cytokeratine 8, 18, 19 |
References: not published | |
