产品报价：询价
更新时间：2014/8/22 10:12:15
产地：美国
品牌：cell-lines-service
型号：COLO-320DM
厂商性质： 生产型,贸易型,服务型,
公司名称： 北京百杰生物科技有限公司
李胜亮 : (13466675923) (13466675923)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | COLO-320DM |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 55 years; Dukes' type C |
| Gender: | Female |
| Tissue: | colon |
| Morphology: | rounded and refractile |
| Celltype: | adenocarcinoma, colorectal |
| Culture Medium: | Ham’s F12 medium supplemented with 2mM L-glutamine and 10% fetal bovine serum (MG-60, CLS order number 820600). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 5 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 11 D13S317: 11 D16S539: 11/12 D5S818: 12 D7S820: 9/12 THO1: 9 TPOX: 8/9 vWA: 15/18 D3S1358: 17 D21S11: 33.2 D18S51: 15 Penta E: 11 Penta D: 9/12 D8S1179: 13 FGA: 20 CLS ? Cell Lines Service, 2010. |
| Isoenzymes: | PGM1,1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A; ES-D, 1 |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
| Products: | serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
References: Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988 | |
