产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：HB-CLS-1
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HB-CLS-1 |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 62 years |
| Gender: | male |
| Tissue: | carcinoma GIII; bladder, urinary |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | etablished from the primary bladder carcinoma grading III of a 62-year-old male |
| Culture Medium: | RPMI 1640 medium supplemented with L-glutamine and 10% fetal bovine serum (MG-70, CLS order number 820700). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10-15 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:8 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 12 D13S317: 12,13 D16S539: 8 D5S818: 11 D7S820: 10,11 THO1:6 TPOX: 8,10 vWA: 15 D3S1358: 14 D21S11: 29 D18S51: 17,19 Penta E: 10 Penta D: 11, 12 D8S1179: 12,14 FGA: 19 CLS ? Cell Lines Service, 2011. |
References: not published | |
