产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：COLO-60H
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | COLO-60H |
| Depositor: | CLS |
| Organism: | Homo sapiens (human) |
| Age/Stage: | 73 years of age |
| Gender: | Male |
| Tissue: | Colon transversum adenocarcinoma. |
| Morphology: | Epithelial |
| Celltype: | Colon adenocarcinoma |
| Culture Medium: | DMEM: Ham’s F12 medium (1:1 mixture) supplemented with 2mM L-glutamine and 5% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Fluid Renewal: | Every 2 to 3 days |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | not tested |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,15 D13S317: 11 D16S539: 9,13 D5S818: 9,16 D7S820: 7.3,10 THO1: 6,9.3 TPOX: 7,10 vWA: 15,16 D3S1358: 15 D21S11: 29,33.2 D18S51: 13,15 Penta E: 11,13 Penta D: 14 D8S1179: 11 FGA: 21,24 CLS ? Cell Lines Service, 2011. |
