产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：HUTU-80
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HuTu-80 |
| ATCC Number: | HTB-40 |
| Depositor: | Larson |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 53 years |
| Gender: | male |
| Tissue: | adenocarcinoma; duodenum |
| Morphology: | epithelial |
| Growth Properties: | monolayer |
| Description: | The cells express receptors for bombesin at up to 6000 sites per cell. |
| Culture Medium: | EMEM supplemented with 2 mM L-glutamine, 1% Non-Essential Amino Acids, 1mM sodium pyruvate and 10% fetal bovine serum (MG-10, CLS order number 820100). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress(1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:5 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice; forms well differentiated papillary adenocarcinoma, (grade I) |
| Karyotype: | (P12) hypodiploid to hyperdiploid with modal number = 46 |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 8,11 D16S539: 10,11 D5S818: 12,13 D7S820: 9,11 THO1: 7 TPOX: 9,11 vWA: 16,18 D3S1358: 15,17 D21S11: 31,32.2 D18S51: 14,17 Penta E: 12,18 Penta D: 2.2 D8S1179: 15 FGA: 21,23 CLS ? Cell Lines Service, 2010. |
| Antigen Expression: | Blood Type B; Rh+ |
| Receptors Expressed: | bombesin |
| Isoenzymes: | PGM3, 1-2; PGM1, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0017 |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Schmidt M et al. Gastrointestinal cancer studies in the human to nude mouse heterotransplant system. Gastroenterology 72: 829-37, 1977. Rousset M et al. Growth-related glycogen levels of human intestine carcinoma cell lines grown in vitro and in vivo in nude mice. J Natl Cancer Inst 65: 885-9, 1980. Lee LT et al. Regulation of the human secretin gene is controlled by the combined effects of CpG mathylation, Sp1/Sp3 ratio, and the E-box element. Molecular Endocrinology 18: 1740-1755, 2005. Ackland ML et al. Synergistic antiproliferative action of the flavonols quercetin and kaempferol in cultured human cancer cell lines. In Vivo 19: 69-75, 2005. | |
