产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：ZR-75-1
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | ZR-75-1 |
| Depositor: | Engel |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 63 years of age |
| Gender: | female |
| Tissue: | Breast (mammary gland); metastatic site: ascites |
| Morphology: | epithelial |
| Celltype: | ductal carcinoma |
| Growth Properties: | adherent |
| Description: | The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene. |
| Culture Medium: | RPMI 1640 medium supplemented with 2 mM L-glutamine, 1 mM Na-pyruvate, 25mM HEPES, and 10% fetal bovine serum (MG-70, CLS order number 820700). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase(1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10-15 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:4 to 1:6 is recommended |
| Fluid Renewal: | 2 to 3 times per week |
| Doubling Time: | 80 hrs |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, forms tumors in nude mice |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 10,11 D13S317: 9 D16S539: 11 D5S818: 13 D7S820: 10,11 THO1: 7,9.3 TPOX: 8 vWA: 16,18 D3S1358: 15,16 D21S11: 31 D18S51: 13,14 Penta E: 7,14 Penta D: 14 D8S1179: 11,13 FGA: 20,22 CLS ? Cell Lines Service, 2011. |
| Receptors Expressed: | Estrogen-receptor +; |
| Isoenzymes: | G6PD, B |
| Products: | mucin (apomucin, MUC-1, MUC-2) |
References: Engel LW et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res 38: 3352-64, 1978. Allegra JC et al. Growth of a human breast cancer cell line in serum-free hormone-supplemented medium. Cancer Res 38: 3823-9, 1978. Allegra JC et al. The effects of 17 beta estradiol and tamoxifen on the ZR-75-1 human breast cancer cell line in defined medium. Eur J Cancer 16: 1007-15, 1980. Allegra JC et al. The regulation of progesterone receptor by 17 beta estradiol and tamoxifen in the ZR-75-1 human breast cancer cell line in defined medium. J Recept Res 2: 17-27, 1981. Dhar MS et al. Protein expression of G-protein inwardly rectifying potassium channels (GIRK) in breast cancer cells. BMC Physiol 6: 8, 2006. Barnard RJ et al. Effects of a low-fat, high-fiber diet and exercise program on breast cancer risk factors in vivo and tumor cell growth and apoptosis in vitro. Nutr Cancer 55: 28-34, 2006. | |
