产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：MDA-MB-436
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | MDA-MB-436 |
| Depositor: | Cailleau |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 43 years of age |
| Gender: | female |
| Tissue: | Breast; mammary gland; pleural effusion |
| Morphology: | pleomorphic with multinucleated component cells |
| Celltype: | Adenocarcinoma |
| Growth Properties: | monolayer |
| Description: | The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining. |
| Culture Medium: | DMEM:Ham's F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine and 10% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add TrypleExpress (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | no |
| Karyotype: | modal number = 45 |
| DNA Profile (STR): | Amelogenin: X,X CSF1PO: 12 D13S317: 10 D16S539: 9 D5S818: 13 D7S820: 10 TH01: 9.3 TPOX: 8 vWA: 14,20 D3S1358: 18 D21S11: 30,31.2 D18S51: 12 Penta E: 10,12 Penta D: 9 D8S1179: 10,14 FGA: 24 CLS – Cell Lines Service 2010. |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 1; ES-D, 1; Me-2, 1; AK-1, 1; GLO-1, 2; Phenotype Frequency Product: 0.0326 |
| Products: | tubulin; actin |
References: Cailleau R et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-5, 1978. Siciliano MJ et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res 39: 919-22, 1979. Brinkley BR et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res 40: 3118-29, 1980. Lan MS et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res 50: 2997-3001, 1990. Kulp KS et al. Essiac(?) and Flor-Essence (?) herbal tonics stimulate the in vitro growth of human breast cancer cells. Breast Cancer Res Treat 98: 249-59, 2006. Srirangam CM et al. Effects of HIV protease inhibitor ritonavir on Akt-regulated cell proliferation in breast cancer. Clin Cancer Res 12: 1883-96, 2006. | |
