产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：MDA-MB-435S
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | HBL-100 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 27 years |
| Gender: | Female |
| Tissue: | Breast origin not clarified |
| Morphology: | Epithelial |
| Growth Properties: | Monolayer |
| Description: | HBL-100 is an epithelial cell line derived by E.V. Gaffney and associates from the milk of a nursing mother and obtained 3 days after delivery. Although there was no evidence of a breast lesion in the milk donor and she had no family history of breast cancer, the karyotype of the recovered cells was abnormal as early as passage 7. This line was able to synthesize a small amount of lactose and would respond to prolactin or estrogen by producing increased amounts of casein. Electron micrographs revealed microvilli, tonofibrils and desmosomes. At passage levels below 35 the line is not tumorigenic in nude mice, but forms colonies in soft agar. Tumorigenicity has been reported to increase above passage 35. The cells contain a tamdemly integrated SV40 genome; it has been reported that they may contain a type D retrovirus that is similar or identical to Mason-Pfizer monkey virus (MPMV). |
| Culture Medium: | Mc Coy’s 5A medium supplemented with 2mM glutamine and 10% fetal bovine serum (MG-200, CLS order number 821200). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number: 800125, 25ml, 800150, 50ml) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 2 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | The stemline chromosome number is near triploid with the modal number of 67 chromosomes, and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q, 11q+, 11q, t(2q;12), t(2q;5q?), t(6p?;16), 16pt and many others are common to most metaphases. Normal chromosomes 11, 14, 15 and 16 are absent; 2, 12, 17 and 19 are monosomic, and the X is disomic. NOTE: According to reports by ATCC, DNA profiling for amelogenin, a sex-chromosome-specific PCR assay that can distinguish X chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining, C-banding, and FISH, with a whole chromosome paint probe to the human Y chromosome. |
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10 D13S317: 12 D16S539: 9,12 D5S818: 11,12 D7S820: 8,12 THO1: 6,8 TPOX: 8 vWA: 16 D3S1358: 14,16 D21S11: 28,30 D18S51: 16 Penta E: 7 Penta D: 12 D8S1179: 12,15 FGA: 25 CLS ? Cell Lines Service, 2010. |
| Antigen Expression: | HLA A1, A10, A11, B7, B8 |
| Receptors Expressed: | Estrogen; prolactin |
| Isoenzymes: | G6PD, B; PGM1, 1; PGM3, 2; ES-D, 1; Me-2, 0; GLO-1, 2; AK-1, 1-2; Phenotype Frequency Product: 0.0008 |
| Reverse Transcriptase: | positive |
| Viruses: | SMRV negative, as confirmed by Real-Time PCR |
References: Gaffney EV, Blackburn SE, Polanowski FP. The hormone response of secreting and nonsecreting humen breast cells in culture. In Vitro 12: 328-329, 1976. Gaffney EV et al. Human serum and the growth of human mammary cells. J. Natl. Cancer Inst. 63(4): 913-918, 1979. Giovanella BC, Gaffney EV, Yim SO, Stehlin JS. Increase of the malignant potential of the human line HBL 100 with long term passage in vitro. In Vitro 16: 211, 1980. Ziche M and Gullino PM. Angiogenesis and neoplastic progression in vitro. J. Natl. Cancer Inst. 69(2): 483-487, 1982. Gaffney EV. A cell line (HBL-100) established from human breast milk. Cell Tissue Res. 227: 563-568, 1982. Caron de Fromentel C et al. Epithelial HBL-100 cell line derived from milk of an apparently healthy woman harbours SV40 genetic information. Exp. Cell Res. 160(1): 83-94, 1985. Marlhens F et al. Karyotype evolution of the human HBL-100 cell line and mapping of the integration site of SV40 DNA. Ann Genet 31(2): 81-86, 1988. Dhaliwal MK, Giovanella BC, Pathak S. Cytogenetic characterization of two human milk-derived cell line (HBL-100) passages differing in tumorigenicity. Anticancer Res 10(1): 113-117, 1990. Laherty RF et al. HBL-100 cells do not secrete casein and lack prolactin and estradiol receptors [letter]. In Vitro Cell. Dev. Biol. 26(10): 933-935, 1990. Faust JB and Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52(9): 2460-2463, 1992. Scolaro C et al. In vitro and in vivo evaluation of ruthenium(II)-arene PTA complexes. J Med Chem 48(12): 4161-71, 2005. Jezierska A et al. ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit 12(8): BR263-276, 2006. | |
