产品报价：询价
更新时间：2023/4/3 18:11:18
产地：美国
品牌：cell-lines-service
型号：BT-474
厂商性质： 生产型,
公司名称： 世联博研（北京）科技有限公司
王荣 : (18618101725) (18618101725)
（联系我时，请说明是在来宝网上看到的，谢谢！）| Designation: | BT-474 |
| Organism: | Homo sapiens (human) |
| Ethnicity: | Caucasian |
| Age/Stage: | 60 years old |
| Gender: | female |
| Tissue: | Breast |
| Morphology: | epithelial |
| Celltype: | mammary gland |
| Growth Properties: | Monolayer; the cells form compact, multilayered colonies; growing slowly and rarely becoming confluent. |
| Description: | The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. |
| Culture Medium: | DMEM:Ham’s F12 medium (1:1 mixture) supplemented with 2 mM L-glutamine, 5μg/ml Insulin and 10% fetal bovine serum (MG-40, CLS order number 820400). |
| Subculturing: | Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37°C for 10 minutes. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. |
| Split Ratio: | A ratio of 1:2 to 1:3 is recommended |
| Fluid Renewal: | 2 to 3 times weekly |
| Freeze Medium: | CM-1 (CLS order number 800125 or 800150) |
| Sterility: | Tests for mycoplasma, bacteria and fungi were negative |
| Biosafety Level: | 1 |
| Tumorigenic: | yes, in nude mice |
| Karyotype: | mode = 55; range = 50 to 112; bimodal shift 58 - 59 and 100 in later passages with 3 marker chromosomes |
| DNA Profile (STR): | Amelogenin: X / X CSF1PO: 10/11 D13S317: 11 D16S539: 9/11 D5S818: 11/13 D7S820: 9/12 THO1: 7 TPOX: 8 vWA: 15/16 D3S1358: 17 D21S11: 28/32.2 D18S51: 13/18 Penta E: 5 Penta D: 9/14 D8S1179: 10/12 FGA: 22/25 CLS ? Cell Lines Service, 2010. |
| Isoenzymes: | G6PD, B; PGM3, 1; PGM1, 1; ES-D, 1; Me-2, 0; AK-1, 1; GLO-1, 1; Phenotype Frequency Product: 0.0426 |
| Viruses: | Test for SMR-Provirus: env-gene negative / gag-gene negative SMRV negative, as confirmed by Real-Time PCR |
| Virus Susceptibility: | mouse mammary tumor virus (RIII-MuMTV) |
References: Lasfargues EY et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J Natl Cancer Inst 61: 967-78, 1978. Lasfargues EY et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-9, 1979. Imai Y et al. Epidermal growth factor receptors and effect of epidermal growth factor on growth of human breast cancer cells in long-term tissue culture. Cancer Res 42: 4394-8, 1982. Kumar R et al. Regulation of phosphorylation of the c-erbB-2/HER2 gene product by a monoclonal antibody and serum growth factor(s) in human mammary carcinoma cells. Mol Cell Biol 11: 979-86, 1991. Littlewood-Evans AJ et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res 57: 5386-90, 1997. Wong LJ et al. AIB1 gene amplification and the instability of polyQ encoding sequence in breast cancer cell lines. BMC Cancer 6: 111, 2006. Isaza-Molina MA et al. In vitro effects of bovine dialyzable leukocyte extract (bDLE) in cancer cells. Cytotherapy 8: 408-14, 2006. Liang Y et al. Vascular endothelial growth factor induces proliferation of breast cancer cells and inhibits the anti-proliferative activity of anti-hormones. Endocr Relat Cancer 13: 905-19, 2006. | |
