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现在位置首页>实验室原料试剂耗材>蛋白纯化>蛋白体分离>截留体积15ml超滤管,Amicon Ultra-15 Centrifugal Filter Units
截留体积15ml超滤管,Amicon Ultra-15 Centrifugal Filter Units
截留体积15ml超滤管,Amicon Ultra-15 Centrifugal Filter Units
  • 截留体积15ml超滤管,Amicon Ultra-15 Centrifugal Filter Units

截留体积15ml超滤管,Amicon Ultra-15 Centrifugal Filter Units

产品报价:询价

更新时间:2012/3/13 11:43:42

地:美国

牌:Millipore

号:多种

厂商性质: 生产型,贸易型,服务型,

公司名称: 上海劢瑞生物科技有限公司

产品关键词: 美国 Teledyne氧电池   美国 Teledyne氧电池  

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产 品 说 明

截留分子量为3K,10K,30K,50K,100K几种。每种包装规格有8个 24个 96个。

  • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
  • High retentate recovery of >90%
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
  • Heat-sealed membrane minimizes downstream extractables
  • 100% integrity tested for reliable performance
  • Convenient sample monitoring with translucent housing and volume gradations
  • Direct pipettor sample access eliminates processing step to recover concentrate
  • High concentration factors of 80–100X

 

Applications

 

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
  • Concentration of dilute or pre-purified proteins from column eluents
  • Desalting, buffer exchange and protein dialysis
  • Size exclusion and protein fractionation

 

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Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery (return to top)

Membrane
NMWL, kDa
Protein Solute Protein Retentate
Volume, mL
Protein Retentate
Recovery, %
3 Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.294 97.5
10 Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.24 93.03
30 BSA, 67 kDa
(1 mg/mL)
0.30 97.94
50 BSA, 67 kDa
(1 mg/mL)
0.17 93.32
100 IgG, 156 kDa
(1 mg/mL)
0.28 89.03
15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).

Specifications (return to top)

Volumes
Maximum Initial Sample Volume, mL* 15
Typical Final Concentrate Volume, µL 200
Centrifugal Force Swinging bucket rotor: 4,000 x g; Fixed angle rotor (35°): 5,000 x g
Materials
Membrane Ultracel regenerated cellulose
Device Housing: Styrene/butadiene
Tube: Polypropylene
Cap and liner: Polyethylene
Dimensions
Active Membrane Area, cm2 7.6
Diameter, mm 29.7
Filtrate tube or vial (centrifuge tube)
Length, mm (with cap and device) 122
Filter unit
Diameter, mm 29.6
Length, mm 72.0
*Except for 35° fixed angle rotor: 12 mL

Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane is CE marked.

 

目录编号 产品名称 NMWL,kDa 数量/包装
UFC900308 装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 3 8
UFC900324 装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 3 24
UFC900396 装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管 3 96
UFC901008 装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 10 8
UFC901024 装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 10 24
UFC901096 装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管 10 96
UFC903008 装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 30 8
UFC903024 装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 30 24
UFC903096 装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管 30 96
UFC905008 装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 50 8
UFC905024 装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 50 24
UFC905096 装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管 50 96
UFC910008 装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 100 8
UFC910024 装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 100 24
UFC910096 装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管 100 96

 

 

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