CYP2J2 is expressed at high levels in cardiac tissue. It is expressed at lower levels in the intestine, liver, kidney, and lung. CYP2J2 is active for the oxidation of arachidonic acid to epoxyeicosatrienoic acids (EETs), which are important for heart and kidney function. CYP2J2 has also been shown to metabolize xenobiotics such as diclofenac and bufuralol1. CYP2J2 is the predominant P450 for the hydroxylation of ebastine and astemizole (non-sedating anti-histamines) in human intestinal microsomes2. More recent studies have shown that CYP2J2 is a high affinity enzyme for the hydroxylation of terfenadine3. Baculovirus-insect cell expressed with supplemental cDNA expressed reductase and cytochrome b5.
• Available size: 0.5 nmole P450 in 0.5 ml
• Representative total protein concentration: 4.0 mg/ml
• Representative P450 content: 210 pmole/mg
• Catalytic assay: Terfenadine Hydroxylase
• Representative catalytic activity: 3,800 pmole/(mg x min)
References
1. Scarborough, P., et.al., Drug Met. Rev., 31:205-234, 1999
2. Hashizume, T. et.al., JPET, 300:298-304, 2002, Matsumoto, S., et.al., DMD, 30:1240-1245, 2002
3. European ISSX, 2003, abstract No. 242
Product Details
- Catalog Number
- 456264
- Brand
- BD Supersomes™
- Name
- Human CYP2J2+OR+B5 0.5ML
- Size
- 0.5 nmole
- Regulatory Status
- RUO
