Filters for AC48、PFC-PRC-SCC

AC48滤波器

AC48腔室使用35x41mm过滤器。

聚碳酸酯轨道蚀刻（PCTE）膜是我们的腔室和仪器系统中使用的主要类型的过滤器。这是一种非常薄的膜，标称厚度在6-10μm之间。这种膜类型具有优异的孔径和密度均匀性，以及其他优点，如低可萃取性，低蛋白质结合和可忽略的吸收。这些特性使其非常适用于趋化性测定以及其他高纯度筛选测定。

PVP处理 - PCTE膜可用润湿剂聚乙烯吡咯烷酮（PVP）预处理，使表面具有亲水性。当介质溶液不能自身润湿膜时，使用PVP处理的膜。这主要发生在使用非常小的孔径或高粘度介质溶液时。某些细胞类型如嗜中性粒细胞不能很好地粘附于PVP处理的膜上，并且在迁移后趋于脱落。虽然在量化过滤器上的细胞时这是有问题的，但是一些使用ChemoTx?系统或MB系列96孔腔室并在板中量化的研究人员发现这是一个优势。细胞外基质蛋白质也不能很好地粘附于PVP处理的膜上。如果您的分析需要这样的涂层，我们建议使用不含PVP的膜。

AP48，AC48，AA12和AA10的过滤器也有硝酸纤维素。这些是更厚（~150μm）的纤维过滤器，有时称为“曲折路径”过滤器。如通常用PCTE膜一样，不是计算已经迁移过膜的细胞，而是在预定孵育后用溶剂澄清这些过滤器，并使用高分辨率光学显微镜观察细胞迁移到过滤器的距离。 。这也被称为“前沿”研究。这些过滤器的建议保质期为一年。它们按订单生产，因此请允许2-3天进行处理。

聚碳酸酯膜有以下孔径和相应的孔密度：

细胞类型以外的许多因素进入孔径选择过程。

Filters for AC48

The AC48 chamber uses 35x41mm filters. Sold in lots of 100.

Polycarbonate track-etch (PCTE) membranes are the primary type of filters used in our chambers and instrument systems. This is a very thin membrane ranging between 6-10μm in nominal thickness. This membrane type offers、 excellent uniformity in pore size and density, as well as other benefits such as low extractables, low protein binding, and negligible absorption. These characteristics make it ideally suited for use in chemotaxis assays as well as other high purity screening assays.

PVP Treatment –PCTE membranes are available pre-treated with the wetting agent polyvinylpyrrolidone (PVP) which makes the surface hydrophilic. PVP-treated membranes are used when the media solutions cannot wet out the membrane by themselves. This occurs primarily when working with very small pore sizes or high viscosity media solutions. Certain cell types such as neutrophils、 do not adhere well to PVP treated membranes and tend to drop off after migrating. While this is problematic when quantifying cells on the filter, some researchers using the ChemoTx? sytem or the MB-series 96-well chambers and quantifying in the plate find this to be an advantage. Extra-cellular matrix proteins do not adhere well to PVP treated membranes either. If your assay requires such coating, we recommend using PVP-free membranes.

Filters for the AP48, AC48, AA12 and AA10 are also available in cellulose nitrate. These are much thicker (~150 μm) fibrous filters, sometimes referred to as “torturous path” filters. Rather than counting cells that have migrated through the membrane, as is commonly done with PCTE membrane, these filters are clarified with solvents after a predetermined incubation, and a high-resolution light microscope is used to observe how far into the filter the cells have migrated. This is also known as a “leading edge” study. These filters have a recommended shelf life of one year. They are cut to order, so please allow 2-3 days for processing.

Polycarbonate membranes are available in the following pore sizes and corresponding pore densities:

Many factors other than cell type enter into the pore size selection process. Please see our page on Experiment Design.