Neuro Probe 12-Well Chemotaxis Chamber、AA12

在 Neuro Probe AA12趋化室中，添加到下部孔中的化学吸引剂作用于添加到上部孔中的悬浮液中的细胞。过滤器将上井和下井分开。孵育后，将过滤器上迁移的贴壁细胞染色并计数。与更常用的AP48腔室相比，AA12具有更大体积的上部和下部井以及更大的暴露过滤器区域。

AA12腔室由精密加工的丙烯酸顶板和底板，装配硬件和硅胶垫圈组成。这些板在两个偏移行中有12个井，每个井有6个井。底板中的每个孔直径为4.75mm，保持150μL，并具有圆形底部。当组装腔室时，顶板中的相应孔形成上部孔。这些顶部孔的体积为100μL。暴露的过滤面积为18mm 2。

将单个25×80mm的过滤膜放置在顶板和底板之间，并将垫圈定位在过滤器上以形成密封。顶部和底部井的配合表面周围的窄凸起边缘增强了密封。

在迁移的细胞可以被染色和计数之前，必须从过滤器的顶侧擦除非迁移的细胞。附件包  （Stock＃P48AP）  包括用于此目的的仪器。

AA12设计用于聚碳酸酯过滤器，但也可以使用硝酸纤维素过滤器。

每个房间都附有 使用和护理的  协议。AA12的过滤器和附件与AP48使用的相同。

In the Neuro Probe AA12 chemotaxis chamber chemoattractants added to the lower wells act on cells in a suspension added to the upper wells. A filter separates the upper and lower wells. After incubation the migrated adherent cells on the filter are stained and counted. In comparison with the more commonly used AP48 chamber, the AA12 has larger volume upper and lower wells and a larger exposed filter area.

The AA12 chamber consists of precision-machined acrylic top and bottom plates, assembly hardware, and a silicone gasket. The plates have 12 wells in two offset rows of six wells each. Each well in the bottom plate is 4.75mm in diameter, holds 150μL, and has a rounded base. Corresponding holes in the top plate form the upper wells when the chamber is assembled. These top wells have a volume of 100μL. The exposed filter area is 18mm2.

A single 25 x 80mm piece of filter membrane is placed between the top and bottom plates, and a gasket is positioned over the filter to create a seal. Narrow raised rims around mating surfaces of top and bottom wells enhance the seal.

Before the migrated cells can be stained and counted, the nonmigrated cells must be wiped off the top side of the filter. An accessory pack (Stock # P48AP) includes instruments for this purpose.

The AA12 is designed for use with polycarbonate filters, although cellulose nitrate filters may also be used.

Each chamber is accompanied by a protocol for its use and care. Filters and accessories for the AA12 are the same as used with the AP48.