MB Series (MBA96, MBB96, MBC96)MB-96、趋化室neuroprobe

MBA96，MBB96和MBC96趋化室是可重复使用的仪器，设计用于一次性96孔微孔板和专门设计的框架式过滤器，两者都适合标准自动读取器。每个腔室由精密制造的可重复使用的顶板和底板组成，铰接并配有弹簧加载的卡子，可以快速准确地组装组件。一个特殊的密封系

顶板有96个通孔; 底板具有单个大凹槽，其尺寸适于容纳微孔板，其形成用于趋化性测定，肿瘤细胞侵袭测定和细胞 - 细胞相互作用研究的底部孔。

神经探针框架过滤器置于微孔板上方。过滤器可以使用粘合带粘附到微孔板的周边，形成一个可以离心的装置。标准形式的荧光，光密度和闪烁读数器可用于计数微孔板中的迁移细胞。许多荧光读数器也可用于计数过滤器上的细胞。

框架式过滤器由聚碳酸酯膜粘合到刚性框架上，使其易于处理，擦拭和染色。过滤器有各种孔径和密度，有或没有粘合剂。

阿  可重复使用的96孔插入件  来代替一次性微板的使用，以及类似的微孔板，插入件可与使用  自动读取器。这些插入件也丙烯酸，并受到同样的  预防措施  在护理和  清洁  的腔室。

保持销将硅胶垫圈固定在腔室顶板的密封表面上; 当垫片夹在板之间时，垫圈形成密封。顶板和底板上的定位销便于板，垫圈和过滤器的对齐。

除硬件外，组装腔室的尺寸为5.75“x 5.00”x 1.5“。

三个MB系列腔室的顶部井容积和暴露的过滤面积不同如下：

* MBC96在上部井中有一个步骤; 如果填充到台阶，则容量为50μL，如果填充到顶部则为225μL。

伴随每个腔室的是  MB系列96孔协议，  用于其使用和护理。

Neuro Probe MBA96, MBB96, and MBC96 chemotaxis chambers are reusable instruments designed for use with disposable 96-well microplates and specially designed framed filters, both of which fit standard automated readers. Each chamber consists of precision-made reusable top and bottom plates, hinged and fitted with spring-loaded catches, enabling rapid and accurate assembly of the components. A special sealing system keeps fluids in place during incubation.

The top plate has 96 through wells; the bottom plate has a single large recess sized to hold a microplate, which forms bottom wells for chemotaxis assays, tumor cell invasion assays, and cell-cell interaction studies.

A Neuro Probe framed filter sets in over the microplate. Filters are available with adhesive strips to adhere to the perimeter of the microplate, forming one unit that can be centrifuged. Standard format fluorescence, densitometric, and scintillation readers can be used to count migrated cells in the microplate. Many fluorescence readers can also be used to count cells on the filter.

The framed filters consist of a polycarbonate membrane bonded to a rigid frame that makes them easy to handle, wipe, and stain. Filters are available in a variety of pore sizes and densities, and with or without adhesive. See 96-well chamber filters for specific information.

A reusable 96-well insert can be used in place of a disposable microplate, and like microplates, the inserts can be used with automated readers. These inserts are also acrylic, and are subject to the same precautions in care and cleaning as the chambers.

Retention pins hold a silicone gasket against the sealing surface of the chamber’s top plate; the gasket forms a seal when sandwiched with the filter between the plates. Registration pins in the top and bottom plates facilitate alignment of plates, gasket, and filter.

Dimensions of the assembled chamber, excluding hardware, are 5.75″ x 5.00″ x 1.5″.

The three MB series chambers differ in top-well volume and exposed filter area as follows:

* The MBC96 has a step in the upper well; capacity is 50μL if filled to the step, 225μL if filled to the top.

Accompanying each chamber is an MB-series 96-well protocol for its use and care. See framed filters for information on their proper use. Filters and other accessories, including replacement gaskets, are available.