PFD-MBSeries、Framed Filters for MB-series 96-well chambers

用于MB系列96孔腔室的带或不带粘合剂的框架式过滤器

*非库存物品，要求提供

聚碳酸酯轨道蚀刻（PCTE）膜是我们的腔室和仪器系统中使用的主要类型的过滤器。这是一种非常薄的膜，标称厚度在6-10μm之间。这种膜类型具有优异的孔径和密度均匀性，以及其他优点，如低可萃取性，低蛋白质结合和可忽略的吸收。这些特性使其非常适用于趋化性测定以及其他高纯度筛选测定。

为了提供适合96孔应用的过滤器，Neuro Probe开发了框架式过滤器，其中过滤膜粘合到刚性框架上，使用户能够在实验室程序期间通过框架保持过滤器。

用于MB系列96孔腔室的框架式过滤器可以使用粘合剂，使过滤器粘附在底部腔室中的微孔板边缘。这允许过滤器和板作为一个单元从腔室中移出，并且如果需要可以放置在微孔板离心机中。

PVP处理 - PCTE膜可用润湿剂聚乙烯吡咯烷酮（PVP）预处理，使表面具有亲水性。当介质溶液不能自身润湿膜时，使用PVP处理的膜。这主要发生在使用非常小的孔径或高粘度介质溶液时。某些细胞类型如嗜中性粒细胞不能很好地粘附于PVP处理的膜上，并且在迁移后趋于脱落。虽然在量化过滤器上的细胞时这是有问题的，但是一些使用ChemoTx?系统或MB系列96孔腔室并在板中量化的研究人员发现这是一个优势。细胞外基质蛋白质也不能很好地粘附于PVP处理的膜上。如果您的分析需要这样的涂层，我们建议使用不含PVP的膜。

聚碳酸酯膜有以下孔径和相应的孔密度：

细胞类型以外的许多因素进入孔径选择过程。

Framed Filters with or w/o adhesive for MB-series 96-well chambers

Sold in boxes of 15.

*non-stock items, call for availability

Polycarbonate track-etch (PCTE) membranes are the primary type of filters used in our chambers and instrument systems. This is a very thin membrane ranging between 6-10μm in nominal thickness. This membrane type offers、 excellent uniformity in pore size and density, as well as other benefits such as low extractables, low protein binding, and negligible absorption. These characteristics make it ideally suited for use in chemotaxis assays as well as other high purity screening assays.

To provide a filter suited to 96-well applications, Neuro Probe developed the framed filter, in which filter membrane is bonded to a rigid frame that enables the user to hold the filter by the frame during laboratory procedures.

Framed filters for the MB-series 96-well chambers are available with adhesive which allows the filter to adhere to the rim of the microplate in the bottom chamber. This allows the filter and plate to be removed from the chamber as a unit and placed in a microplate centrifuge if desired.

PVP Treatment –PCTE membranes are available pre-treated with the wetting agent polyvinylpyrrolidone (PVP) which makes the surface hydrophilic. PVP-treated membranes are used when the media solutions cannot wet out the membrane by themselves. This occurs primarily when working with very small pore sizes or high viscosity media solutions. Certain cell types such as neutrophils、 do not adhere well to PVP treated membranes and tend to drop off after migrating. While this is problematic when quantifying cells on the filter, some researchers using the ChemoTx? sytem or the MB-series 96-well chambers and quantifying in the plate find this to be an advantage. Extra-cellular matrix proteins do not adhere well to PVP treated membranes either. If your assay requires such coating, we recommend using PVP-free membranes.

Polycarbonate membranes are available in the following pore sizes and corresponding pore densities:

Many factors other than cell type enter into the pore size selection process. Please see our page on Experiment Design.