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（联系我时，请说明是在来宝网上看到的，谢谢！）V01003必拓生物pET30a(+)载体，pET30a(+) vector，质粒载体图
N-末端His6标签，S.tag
成分:10 mM Tris（pH8.0）
保存条件:-20℃保存
产品目录
| 货号 | 产品名称 | 规格 | 报价(元) |
| V01003 | pET30a(+) vector | 10μl，20ng/μl | 600 |
pET30a(+)载体，pET30a(+) vector，质粒载体图
LOCUS pET-30a(+) 5422 bp DNA circular 16-DEC-2016
DEFINITION pET-30 a (+).
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
misc_feature complement(1017..1832)
/vntifkey="21"
/label=kan
misc_feature complement(3975..5054)
/vntifkey="21"
/label=lacI
promoter 23..41
/vntifkey="30"
/label=T7\promoter
rep_origin 1938..2557
/vntifkey="33"
/label=pBR322\Ori
BASE COUNT 1282 a 1457 c 1398 g 1285 t
ORIGIN
1 atcgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
61 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat atgcaccatc
121 atcatcatca ttcttctggt ctggtgccac gcggttctgg tatgaaagaa accgctgctg
181 ctaaattcga acgccagcac atggacagcc cagatctggg taccgacgac gacgacaagg
241 ccatggctga tatcggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag
301 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
361 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
421 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
901 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc
961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag
1021 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
1081 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
1141 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
1201 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
1261 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
1321 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
1381 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
1441 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
1501 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
1561 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
1621 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
1681 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
1741 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
1801 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
1861 gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
1921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
1981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
2041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
2101 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
2161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
2221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
2281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
2341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
2401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
2461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
2521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
2581 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
2641 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
2701 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
2761 cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag
2821 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
2881 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
2941 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
3001 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
3061 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
3121 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
3181 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
3241 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
3301 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
3361 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
3421 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
3481 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
3541 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
3601 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
3661 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
3721 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
3781 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
3841 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
3901 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
3961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
4021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
4081 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
4141 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
4201 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatgtccgca
4261 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
4321 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
4381 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
4441 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
4501 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
4561 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
4621 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
4681 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
4741 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
4801 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
4861 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
4921 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
4981 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
5041 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
5101 ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga
5161 ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag
5221 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
5281 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
5341 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
5401 gcgtccggcg tagaggatcg ag
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