产品报价：询价
更新时间：2017/3/23 11:53:28
产地：美国
品牌：GE
型号：28-9355-97
厂商性质： 生产型,贸易型,服务型,
公司名称： 上海基星生物科技有限公司
上海基星生物科技有限公司 : (13817375983) (021-50276558)
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| 28-9355-97 | Dextrin Sepharose High Perform 25ml |
| 28-9355-98 | Dextrin Sepharose High Perform 100ml |
| 28-9356-00 | StrepTactin Sepharose High Per |
| 28-9805-82 | MembraneProteinPurificationKit |
| 17-5468-15 | HiScreen Capto SP ImpRes |
| 17-5470-15 | HiScreen Capto Q ImpRes |
| 17-5474-15 | HiScreen MabSelect SuRe LX |
Dextrin Sepharose High Performance and MBPTrap HP 1 ml and 5 ml columns for affinity purification of recombinant proteins tagged with maltose binding protein
Dextrin Sepharose High Performance and prepacked MBPTrap HP columns provide convenient affinity purification of recombinant proteins tagged with maltose binding protein (MBP). Dextrin Sepharose High Performance has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose High Performance based medium enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
SDS-PAGE (reducing conditions) of the purification of MBP2*-paramyosin-d-Sal.
Automated purification of MBP2*-paramyosin-d-Sal using the two-step AC-GF protocol on MBPTrap HP 1 ml (AC) and HiLoad 16/60 Superdex 200 pg (GF).
| TECHNICAL SPECIFICATIONS | |
| Matrix | Rigid, highly cross-linked 6% agarose |
| Average particle size | 34 μm |
| Ligand | Dextrin |
| Dynamic binding capacity1 | Approx. 7 mg MBP- DSal/ml medium |
| Approx. 16 mg MBP-bGal/ml medium | |
| Chemical stability | Stable in all commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning) |
| pH stability | |
| working | > 7 |
| short term | 2¾13 |
| Storage | 4°C to 8°C in 20% ethanol |
| MBPTrap HP | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1ml); 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate2 | 1 ml/min (1 ml); 5 ml/min (5 ml) |
| Max. flow rate2 | 4 ml/min (1 ml); 20 ml/min (5 ml) |
| Max. pressure2 | 0.3 MPa, 3 bar |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 8°C |
| 1 Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-DSal (MBP2*-paramyosin-DSal, Mr approx. 70 000, multimer in solution), and MBP-bGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution) 2 H20 at room temperature |
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http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=167322
